Effects Of Different Doses Of Vitamin A And D3 On Immune Cell Activity In Rats

Objective To study the effect of vitamin A (VA) and vitamin D (VD3) supplementation on cellimmune function in rat.Methods1’. The study on the function of rats immune cells50Wistar rats, early delectation, maleand female half and half, were randomly divided into5groups: control group, VA,10VD3and VA+VD3and50VD3groups. VA group was given1390IU VA/Kg body weight per day;10VD3groupwas given200IU VD31Kg body weight per day, VA+VD3group was given1390IU VA+200VD3IU/Kg body weight per day,50VD? group was given1,000IU/kg VD3per day (for50times ofphysiological dose).while the control group received the same volume of vegetable oil’the dosage areall vegetable oil1ml/Kg by their body weight. Basic diet was given to all the four groups whichcontained VA278IU/Kg.d,VD320IU/Kg.d, both were rats’ physiological doses. The experimentlasted4weeks. Cell counting kit (CCK-8) method was used to detect lymphocyte proliferation andflow cytometry was used to detect monocyte fluorescence intensity atfer labeled by CD-14.Results.1. Atfer supplementation for four weeks, lymphocyte proliferations in VD3group and VA+VD? group increased dramatically, accompanying the value of OD rose0.4K0.22respectivelycomparing with the control group(p <0.05); however, no signiifcant change was observed betweenVA group and the control group (p>0.05) and no synergistic effect existed between vitamin A andvitamin D3by using interactive analysis. In the phagocytosis activity detection of monocytes labled byCD-14, the values of fluorescence intensity increased3,65，4.91，4.50in VA group.10VD3group andVA+VD3group compared with the control group, respectively(p<0.05), which indicated that thephagocytosis activities of mononuclear cell in each supplementation group obviously were enhancedsignificantly, while no significant differences exsisted among the supplementation groups (p>0.05).Also, no synergistic effect existed between vitamin A and vitamin D3.2.After four-week replenishment of VD3, OD value of proliferative activity in10VD3grouplymphocytc, which is obviously higher than2.64(p<0.05) of the control group.However, there is no remarkable difference between50VD3group and the control groupon proliferative activity of lymphocyte (P>0.05). Adopt FCM to observe the phagocyticindex (lfuorescene intensity) of monocytes. The results show that the proliferative activity&fluorescene intensity of10VD3group rats’ monocytes is19.98, which is clearly higherthii：】15.48in the conia>! Group. However, the proliicrative d:vity&lfuorescenei’ntcjisih of50VD; group rats’ monocyte: replenish乂i by high dose oi v u\iv\n VI). is notshown on obvious enlL/’cemcnt. Conclusion Supplementation of Vitamin A that is5-fold of the physiological requirement or (and)10-fold vitamin D3could enhance the lymphocyte proliferation and the phagocytosis activity ofmonocytes in rats， and there is no interaction effect between vitamin A and vitamin D3. However,too high dose (50times of physiological dose) has not been observed on remarkableimmune regulation. Further research needs to be carried out.