Study Of Liver-Associated Lymphocytes In Hepatitis B

Liver-Associate Lymphocytes(LAL)encompassesall sinusoidallymphocytes ,be used for this fourth sinusoidal type. We set up a methodfor observed LAL of rat's by electron microscope ( EM ). Weconcluded that LAL was more frequent in periportal areas. Largegranular lymphocytes ( LGL ) was one special type of LAL thatexpression of dense granules and 0.2um vesicles ( rod- cored and"empty" vesicles ). There was significantly correlation between thenumber of 0.2um vesicles and that of dense granules of hepaticLGL(P<0.Ol).We examined CD3ICD56 expression of liver lymphocytes from32 liver biopsy specimens obtained from patients with hepatitis B(RB) by two- color flow cytometry. It has shown that human liverlymphocytes differ phenotypically from peripheral blood lymphocytes( PBL ). In acute FIB, the number of lymphocytes increased. NKcell play an important role ( P< 0.05 ). Between the number ofCD56+ cell and that of CD3+CD56- cell no difference in chroniclight FIB. In chronic moderat or severe HB, CD3+CDS6- cell playan important role ( N 0.05 ). These results shown that LAL madeup and biological effect have difference in different hepatitis B.We examined CD56 antibody and Fas ligand ( FasL ) antibodyexpression on paraffin - embedded sections from patients with HB byimmunocytochemical staining. FasL protein were observed both inLiver- infiltrating mononuclear cells and in cytoplasm of hepatocytes.The expression of FasL were closely linked to the degree of liverlesion ( N 0.01 ). CD56 positive cells show spot of distribution oninfiltrating lymphocytes in portal or periportal areas, especially nearthe edges of the piecemeal necrosis region or infiltratinglymphocytes. We concluded that FasIFasL system was involved inthe mechanism of hepataegte apoptosis of FIB. liver biopsies from40 patients with FIB were observed by EM. LAL was divided typicalPit cells, fixed lymphocytes and small lymphocytes. These results shownthat LAL biological activity and expression of morphology have2difference in different of RB. To sum up , we concludedthat the process of LAL resist RB virus was very complicated.