| T helper 1,2 phenotype cytokines from peripheral blood of the patients with chronic hepatitis B virus infection and chronic hepatitis C virus infection were detected, the expressions of IFN- y -, IL-6 on T lymphocyte member, and T cells subpopulation were investigated, from which the cellular and molecular immunoregulation from patients with CHB and CHC could be realized, and differences between CHB and CHC be explored.1. The serum levels of IFN-y ^ IL-2 > IL-4^ IL-6 and EL-12, by using enzyme-linked immunosorbent assay in this study, 35 individuals with chronic HCV infection, 30 chronic HBV infection, 20 healthy subjects as normal controls were observed. The results are: Th2 cytokines(IL-4 and IL-6) were significantly increased hi chronic HCV\ HBV infected patients as compared with normal controls(P<0.05). Similarly, the level of Thl cytokine(IL-2) was also elevated in individuals with chronic HCV\ HBV infection when compared with normal controls^O.OS). However, the levels of Thl cytokine(EFN- y )and regulator(IL-12)were slightly increased, but no differences between chronic HCV\ HBV infection and normal controls were found(P>0.05). All cytokine levels exist no differences between CHB and CHC. We draw conclusions that the levels of Th2 cytokines were higher than that of Thl cytokine in HCV and HBV infection. Thus, there exist a low ratio of Thl / Th2 in chronic HCV and HBV infected patients and an enhanced Th2 responses during HCV and HBV infection, which may partly be responsible for the persistence of HCV and HBV infection.2. The results obtained from T lymphocyte subsets of peripheral blood, 36 chronicHCV infection, 41 chronic HBV infection, by fluorescence activated cell sorting (FACS) were: (1) CD/ T cells were decreased and the CD//CDg+ratio fallen markedly with CHB as compared with healthy individuals(P<0.01); there is no difference in CD/ T cells with CHB as compared with healthy individuals (P>0.05). (2) CD/ T cells were decreased and the CD//CD/ ratio fallen markedly, while percent of CD/ T cells increased significantly with CHC as compared with healthy individuals, respectively(P<0.01). (3) CD/ T cells were decreased and the CD//CD/ratio fallen markedly, while percent of CD/ T cells increased significantly with CHC as compared with CHB(P<0.05). The results indicated that the disturbance of immunoregulation with CHB> CHC was obvious. We have probably a good reason explained the causes of high chronicity with CHC because of the decrease in CD/ T cells and CD//CD/ ratio and the increase in CD8+ T cells with CHC as compared with CHB.3. The results from studies on the activities of IFN- Y and IL-6 of periperal blood T lymphocytes member from healthy individuals, patients with CHB and CHC by indirect immunofluorescent antibody method(IFAT) were: (1) The activities of IFN- Y and IL-6 induced by periperal blood T lymphocytes may be measured in patients with CHC> CHB and Normal.(2) The activity of IL-6 from healthy individuals was lower than that of patients with CHB^ CHC. (3) The activity of IFN- Y on member of periperal blood T lymphocyte from patients with chronic HBV, HCV infected patients was low abruptly, but there was no difference between CHB and CHC, from which we learned that it was this low activity of IFN- Y that maybe disturb the elimination of HBV-. HCV.In conclusion, (1) cytokines produced by Thl cells and CD//CD/ ratio were decreased in patients of CHB and CHC. (2) there were not only evident temporary abnormalities of cytokine proportion in serum but also a low activity of IFN- Y on members periperal blood T lymphocytes from patients with chronic HBV, HCV infected patients, particularly CHC. (3)although there were no differences on the levels of T helper 1,2 phenotype cytokines between CHB and CHC, the percent of CD/ cells and ratio of CD//CD/ by fluorescence activated cell sorting(FACS) were decreased in chronic HCV infected patients as compared with chronic HBV infected patients, which probably resulted from the low titer HCV in blood that may trigger the insensitive...
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