The Effects Of Vitamin D On Interferon-α JAK-STAT Signaling Pathway In Peripheral Blood Mononuclear Cells Of Chronic Hepatitis B Patients

Objective To investigate the influence of vitamin D(VD)and Interferon-α(IFN-α)on the expression of JAK-STAT signal transduction molecules and antiviral protein in PBMCs of chronic hepatitis B patients.Methods We collected 63 chronic hepatitis B patients and 23 healthy volunteers from liver disease department outpatient of the second Affiliated Hospital of Anhui Medical University from February 2015 to January 2016.The peripheral blood mononuclear cells(PBMCs)had been separated from peripheral blood by density gradient centrifugation,cultivated and then divided into control group,IFN-α group,VD group and combined treatment group,which were treated with different concentrations of IFN-α or VD.We investigated moleculars of JAK-STAT signal transduction pathway and the antiviral protein(STAT1、STAT2、IRF9、Mx A)expressions through western blot method,thus investigating the effect of VD on the expression of JAK-STAT signal transduction Pathway to IFN-α in PBMCs of Chronic Hepatitis B patients.Results Compared to control group,when treated with different concentrations of IFN-α,the expression of JAK-STAT signal transduction molecules STAT1,STAT2,IRF-9 and anti viral protein Mx A in IFN-α groups increased obviously.And the molecules was expressed to the highest level when treated with 400 IU/ml IFN-α(P<0.05).And compared to control group,when treated with different concentrations of VD,the expression of JAK-STAT signal transduction molecules and anti viral protein in VD groups increased obviously,and the highest expression was in 0.1nmol/ml VD group(P<0.05).Compared to IFN-α group,the expression of JAK-STAT signal transduction molecules and anti viral protein in joint treatment of IFN-α and VD group increased significantly(P<0.05).And compared to healthy controls,the expression of JAK-STAT signal transduction molecules and anti viral protein in PBMCs of CHB patients decreased significantly(P<0.05).Conclusion The combined effects of VD and IFN-α would significantly up-regulates the expression of JAK-STAT signal transduction pathway molecules and antiviral protein in PBMCs of patients with hepatitis B,thus VD may enhance the antiviral effect of IFN-α.