To Study The Expression Of P16, CyclinD1 And Their Relationship In Ovarian Carcinoma

Objective: To study the expression of P1 6, CyclinD1 and their relationship in ovarian carcinoma.Methods: To examine the expression and condition of P16 and CyclinDI in ovarian carcinoma and normal ovarian tissue using immunohistochemistry and in situ hybridization.Results:1. P16 was low expressed in ovarian carcinoma of 68 cases. 20 cases were high expression (29.4%) (++~+++) , 16 cases weakly positive (23.5%) ( + ) and 32 cases negative(47.1%) (-).2. CyclinD1 was high expression in ovarian carcinoma of 68 cases. 36 cases were high expression (52.9%) (++?++) which were only in cancer cells, 28 cases were undertectable(41.2%) ( + ) and 4 cases were negative(5.9%)(-) in 68 cases of samples.3. The mRNA was pansy particle in cell nucleus and/or cell plasma in the results of examining by the methods of in situ hybridization. P16 were positive by both immunohistochemistry and in situ hybridization examination and the number and distribution of positive cells was parallel in 36 cases of samples; But 2 cases of p16 were negative by immunohistochemistry while its cDNA梞RNA were postive by in situhybridization examination. CyclinD1 were positive by both immunohistochemistry and in situ hybridization in 64 cases of samples, and 4 cases were negative by both immunohistochemistry and in situ hybridization examination in these samples.4. Conclusion:1. The expression of p16 was law in ovarian carcinoma than in normal ovarian tissue, and the expression of CyclinDl was higher in ovarian carcinoma than normal ovarian tissue.The low expression of P16 and the high expression of CyclinD1 play a collaborative role in the development.mechanism of ovarian carcinoma.2. There is a negative relation between expressions of P16 and CyclinDl in ovarian carcinoma.Both the absence and mutation of p16 were important in the development of ovarian carcinoma.3. In ovarian carcinoma, the CyclinD1 mRNA and protein were low expressed or over expressed, which indicated that the mechanism of the over expression of CyclinD1 gene.