Experimental Study On The Dopamine Synthesis And Metabolism Of Wendan Decoction And Its Compositions

Objective:To investigate the indication of WenDan Decoction (WD) for SchizoPhrenia,this research focuses the synthesis and metabolism ofDopamine (DA) in rats striatum .Methods:We have observed the effects of WD and its compositions on the stereotyped behaviors of rats. Immunohistochemistry technology was used to evaluated tyrosine hydroxylase (TH) of DA synthesis. DA, 5-hydroxytryptophan(5-HT) and their respective metabolic products 3,4 -dihydroxyphenylacetic (DOPAC), homovanillic (HVA) are also precisely quantified in this research by using high-pressure liquid chromatography with electrochemical detector(HPLC-EC). The main metabolism monoamine oxidase (MAO) of DA was processed too. RESULTS:WD can increase the synthesis and the metabolism process of DA .It is resulted in that WD can cont -rol DA bonding D2. The composition regularity of WD is that expellingphlegm plus regulating the flow of Qi(EP-RFQ) and invigorating the spleen to clear away damp pathogen plus regulating the flow of Qi (ISCADP-RFQ) can mainly accelerate DA synthesis, expelling phlegm plus invigorating the spleen to clear away damp pathogen(EP-ISCADP) can mainly increase the metabolism process of DA.