| Objective: The incidence of coronary heart disease (CHD) is increasing gradually. CPID had been a main fatal disease that influenced mankind health. To study its pathology, pathogenesis and control the risk factors is an important task nowadays for medical study. It was considered that occurrence and development of CHD is because of plaque rapture at the basis of coronary artery atherosclerosis, leading to coronary artery stenosis even total occlusion. The dysfunction of endothelium, smooth muscle cell proliferation, activation of blood platelet and thrombosis embolism are main mechanisms. Homocysteine is a new risk factor of CHD, which differ from other conventional risk factors such as smoking, sex, age, blood pressure, diabetes , lipid disorders. However, the mechanism of HCY inducing CHD is not demonstrated clearly. The aim of this study is to investigate the relationship between HCY and stability of coronary lesions, severity of coronary stenosis, furthermore, explore the mechanisms by measuring the levels of HCY, NO, vWF, and IL-8 in patients with coronary heart disease.Methods: The study group comprised 96 consecutivepatients who underwent coronary angiography in cardiac center of the people's Hospital of HeBei Province, including 56 patients with unstable angina pectoris, 40 patients with stable angina pectoris. At the same time 20 normal patients with normal angiography served as controls.Exclusion criteria: acute myocardial infarction within four weeks, left ventricular ejection fraction<30%, valvular heart disease, recent operation and injury, renal or liver dysfunction, acute or chronic inflammation, acute leukemia, acute cerebrovasular disease, bronchial asthma, von Willebrand disease, cancer, oral vitamins, lack of estrogen, organ transplantation, self immunity disease.Age, sex, hypertension, diabetes, TG, TC, HDL-C, LDL-C, VLDL-C, smoking and CHD family history in every subjects were recorded in detail.Blood sampling: Peripheral blood samples were taken before angiography respectively. Coded samples were stored at -80"C and analyzed in a single batch for the study, thus, patient management was independent of these results. Levels of HCY, IL-8 and vWF were measured with ELISA. Plasma nitric oxide was measured by nitrate reductase.Statistics analysis: SPSS 10.0 software pack was used to make statistical-analysis. Initially the homogeneity of variance between all the groups was analyzed. All the numerical data was shown as mean + standard deviation and students t test was used to establish significance. Chi-square test was used for analysisof categorical data. Linear Correlation analysis was used to measure the coefficient of correlation about the correlation data. We took p<0.05 as statistic significance level. Results1 There was no statistical difference among each studied group about clinical features .2 The comparisons of HCY > NCK vWF and IL-8 levels in each groups.2.1 The levels of HCY vWF and IL-8 were significantly higher in UAP groups than those in SAP group, (20.42+ 5.89 Mmol/1 vs 14.22+ 4.50Mmol/l , p<0.01), (137.94 + 45.12% vs 103 + 16.48%, /K0.01 ) ; ( 101.93 + 21.23 Mmol/1 vs 69.91 + 20.15Mmol/l, p<0.0\) , and they were significantly higher than those in control group, (9.98 + 2.95Mmol/l, p<0.01); ( 75.69 + 19.22%, p<0.0\) (43.98 +15.02Mmol/l, p<0.0\); The level of NO was significantly lower in UAP group than those in SAP group and control group (55.93 + 23.90Mmol/l vs 83.90 + 36.70Mmol/l,106.03+ 31.65Mmol/l,/?<0.01)o2.2 The levels of plasma HCY in patients with triple-vessel or double-vessel lesions were significantly higher than those with single-vessel lesions (21.75 + 6.23Mmol/1, 18.24 + 6.17Mmol/l vs 14.82 +4.0lPmol/l /?<0.01) . The plasma level of IL-8 were significantly different among three groups (108.89 + 22.29Mmol/l), (90.59+ 12.59Mmol/l), (73.04 + 24.03Mmol/1),(/?<0.01) .With the increasing numbers of coronary artery lesions, there was a trend that the level of NO decreased and the...
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