| Objective: Adiponectin ( APN ) is a newly detected adipose-specific plasma protein and abundant in blood stream of normal people. Recent investigations have indicated that adiponectin related to several facets of the atherosclerosis by suppressing expression of endothelial cell adherence factors, proliferation of smooth muscle cell, differentiation of macrophages to foam cells, termination inflammatory reaction, resisting atherosclerosis, protecting endothelial cell, and so on. In addition, Further investigations will improve recognition on occurrence and development of atherosclerosis and provide a new pathway prevent atherosclerosis. In present, investigations have indicated that the plasma adiponectin levels in patients with coronary heart disease were lower compared with those with healthy subjects. But the relationship between adiponectin and endothelial markers of plasma endothelin-1 ( ET-1 ), Nitric oxide ( NO ), von Willebrand factor ( vWF ) has been researched less. In this experiment, the plasma levels of adiponectin, ET-1, NO and vWF in patients with coronary heart disease were researched. To detect the relationship between adiponectin and the endothelial markers of plasma ET-1, NO, vWF. Methods: 60 patients with coronary heart disease ( CHD ), who came from the inpatients of 2th hospital affiliated with the HEBEI Medical University ( 47 males and 13 females ), with average age of 52.4±8.0 years. The patients with CHD were divided into two groups : the stable angina pectoris ( SAP ) group ( 29 males and 6 females ) with average age of 52.8±7.2 years and the acute coronary syndrome ( ACS ) group ( 18 males and 7 females ) with average age of 51.8±9.1 years. The diagnostic criteria of CHD, SAP and ACS were based on"internal medicine of the sixth edition"and coronary angiography. Another 28 healthy people were in control group ( 20 males and 8 females ), with average age of 49.5±7.6 years. They were ruled out CHD through electrocardiogram exercise test or coronary angiography. All subjects not only had excluded body mass index ( BMI )≥25kg/m2 and <18.5kg/m2, but also had no other diseases such as Cerebrovascular disease, Main Artery disease, Liver and Kidney disease, Metabolic disease ( Diabetes, Hyperthyreosis ). The age, sex , BMI showed no difference among three groups. Taking ulnar venous blood 5ml on an empty stomach, infusing anticoagulation tube, using centrifugal machine, 3000 r /min, centrifuging 10min, the plasma in Eppendorf was preserved in -80℃refrigerator. Plasma APN and vWF were detected by enzyme-linked immuneosorbent assay ( ELISA ), while the levels of ET-1 were detected by radioimmunoassay ( RIA ). The plasma NO levels were detected by enzymatic assay with nitrate reductase. The relationship between adiponectin and the endothelial markers of plasma ET-1, NO, vWF was analyzed.Results: Adiponectin and NO levels in plasma in patients with CHD [ ( 7.79±3.22 ) mg/L, ( 41.57±14.73 )μmol/L] were significantly lower than those in healthy control group [ ( 11.35±2.90 ) mg/L ( 67.51±18.69 )μmol/L, ( P<0.01 ) ], while ET-1 and vWF levels [ ( 81.65±23.07 ) pg/mL, ( 119.3±54.59 )% ] were significantly higher than those in healthy control group[ ( 52.17±13.65 ) pg/mL ( P<0.01 ), ( 96.45±32.10 )% ( P<0.05 ) ]. The plasma adiponectin and NO levels in ACS group were significantly lower than those in SAP group[( 6.66±3.10 ) mg/L vs ( 8.60±3.09 ) mg/L ( P<0.05 ); ( 35.73±15.82 )μmol/L vs ( 45.74±12.53 )μmol/L ( P<0.01 )], while the plasma ET-1 and vWF levels were significantly higher [( 89.49±25.53 )pg/mL vs ( 76.05±19.65 ) pg/mL ( P<0.05 ) , ( 136.63±53.94 )% vs ( 106.95±52.35 )% ( P<0.05 ) ]. The level of adiponectin was negatively correlated with that of ET-1 ( r=-0.522, P<0.01 ) and positively correlated with that of NO in plasma ( r=0.415, P<0.01 ), while adiponectin was irrelated with vWF ( r=-0.129, P>0.05 ). The level of NO was negatively correlated with that of ET-1 (r=-0.406,P<0.01 ) and vWF in plasma ( r=-0.285,P<0.05).Conclusion: 1 The plasma levels of adiponectin and NO decreased significantly in patients with coronary heart disease , while those of ET-1 and vWF increased significantly ; 2 The plasma levels of adiponectin and NO decreased in patients with ACS compared with SAP, while those of ET-1 and vWF increased ; 3 The level of adiponectin was negatively correlated with that of ET-1 and positively correlated with that of NO in plasma, while adiponectin was irrelated with vWF .
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