Experimental Study Of "Qixiang" Aerosol On Toxicological Assessment And The Immunological Function In Mice

Objective:The stability and toxicology of "Qixiang" aerosol was studied to evaluate its stability and safety. To preliminary study the effect of "Qixiang" aerosol immunological function in mice. Methods:1. To inspect stability of sample, the contents of astragalin was tested by Accelerating experiment. To inspect acute toxicity by mice LD₅₀ and effect of mutagenesis by Ames test, sperm shape abnormality test in mice and bone marrow cell test.2. Mice were randomly divided into normal control group, Cy group(Using the model of immune function in low level induced by cyclophosphamide), Cy +"Qixiang" aerosol group, "Qixiang" aerosol group. To Measure the index of Thymus and Spleen in mice, the phagocytic index of mononuclear macrophage(carbon granular clearance test), the Cytotoxic activity of splenic NK cell in mice, the half number hemolysis rate in serum mice(HC₅₀), T-lymphocyte subsets in peripheral blood, the contents of cytokine IL-2 induced by splenic cell, the contentsof TNF-a induced by peritoneal macrophage. Compared and analyzed each group.Result:1. Acute toxicity test showed LD₅₀ >10.0g/kg.bw. Ames test and micronucleus test of bone marrow cell showed the sample of "Qixiang" aerosol was safe without mutagenesis. No toxicity was observed in sperm shape abnormality test in mice.2. "Qixiang" aerosol can promote obviously the phagocytic index of mononuclear macrophage and the Cytotoxic activity of splenic NK cell to raise nonspecific immunological function in immunosuppressive mice and have no obvious effect of nonspecific immunological function in normal mice.3. Compared with Cy group, "Qixiang" aerosol can significantly raise the half number hemolysis rate in serum mice(HC₅₀) in Cy+"Qixiang" aerosol group. There is no obvious effect of humoral immune function in normal mice.4. Compared with Cy group, "Qixiang" aerosol can significantly promote T-lymphocyte function, the ability of IL-2 production by mice splenocytes, decrease the content of TNF-a induced by peritoneal macrophage in Cy+"Qixiang" aerosol group. There is obvious effect of cellular immune function in mice.