Studies On Immunologic Regulation Effects Of D-α-Tocopherol Polyethylene Glycol 1000 Succinate In Mice

As an important immunopotentiator,vitamin E has the ability of improve immune function and reproduction,anticancer and antiaging,etc.It's widely used in many fields such as food antioxidant,nutritious additive,medicine and cosmetic products.But in view of its lipid solubility, the absorption in human body needs bile acid and oil,its application in food industry is greatly limited.As the most active water-soluble derivative of vitamin E,D-α-tocopherol polyethylene glycol 1000 succinate(TPGS) solves the problem quite well.In overseas,TPGS is widely used as solubilizer,plasticizer,emulsifier,absorption enhancer,and vector of poorly-soluble drugs delivery system,which can enormously increase the absorption rate.Our study set up an animal model using the novel nutritional supplement of vitamin E——TPGS,on the basis of RDA of healthy adults 15 mg/60 kg,the mice were randomly divided into five groups according to the equivalent dosage,group B～C received agavage of 1,5,10 and 20 times dosage with group A as control.Via determination of T lymphocyte subsets by FCM,serum hemolysin experiment,CRBC phagocytosis of macrophage cell experiment and determination of cell apoptosis by agarose gel electrophoresis,combined with determination of body weight and immune organ index,comprehensively discussed the immunological regulation effects of TPGS on growth condition,immune organ development,cellular immunity,humoral immunity,nonspecific immunity and immune cell apoptosis,so as to provide sufficient theoretical basis of optimal dosage of TPGS as immunopotentiator.Results are as follows:(1) After thirty days of administration,the gain of body weight and immune organ index of every group had no significant difference(P＞0.05).(2) TPGS had a regulation effect on CD4~+T(%),which was dual-directional and dosage dependent.It meaned CD4~+T(%) increased continuously at 1 time and 5 times dosage,both were higher than the control group extremely significantly(P＜0.01),when it comed to 10 times dosage, CD4~+T(%) began to decrease,but still a little higher than the control group(P＞0.05),when it comed to 20 times dosage,it was lower than the control group significantly(P＜0.05).CD8-T(%) of every administration group raised up with no significant difference comparing to the control group(P＞0.0.5).CD4~+T/CD8~+T was higher than the control group at 1～10 times dosage,and reached its peak at 5 times dosage(P＜0.05),when it comed to 20 times dosage,TPGS appeared inhibition,but not significant(P＞0.05).(3) TPGS had a regulation effect on serum hemolysin content in mice.It meaned serum hemolysin content increased continuously at 1 time and 5 times dosage,both were higher than the control group extremely significantly(P＜0.01),when it comed to 10 times dosage,it began to decrease,but still a little higher than the control group(P＞0.05),when it comed to 20 times dosage, TPGS appeared significant inhibition(P＜0.05).(4) TPGS had a regulation effect on phagocytosis percentage.It meaned phagocytosis percentage increased continuously at 1 time and 5 times dosage,both were higher than the control group extremely significantly(P＜0.01),when it comed to 10 times dosage,phagocytosis percentage declined,but still a little higher than the control group(P＞0.05),when it comed to 20 times dosage, TPGS appeared significant inhibition(P＜0.05).Phagocytosis index had a similar change trend.(5) Determined apoptpsis in thymus cell using agarose gel electrophoresis,DNA Ladder in every administration group weakened comparing to the positive control group,5 times dosage was the most obvious,followed by 1 time dosage,10 and 20 times dosage showed no obvious difference.The results showed that TPGS had no significant influence on the gain of body weight and immune organ index,but could improve cellular immunity,humoral immunity and nonspecific immunity in different extent,it also inhibited thymus cell apoptpsis induced by dexamethasone. The immunologic regulation effect of TPGS in mice was closely related to its dosage,as a safe and effective immunopotentiator,the optimal dosage of TPGS was 1.25 mg/kg·d.