Synergistic Effect Of-174 G/C Polymorphism Of The Interleukin-6 Gene Promoter And 469 E/K Polymorphism Of The Intercellular Adhesion Molecule-1 Gene In Patients With Cerebral Infarction

Objective: Intercellular adhesion molecule-1 (ICAM-1) and interleukin-6 (IL-6) are involved in the pathogenetic mechanisms responsible for cerebral infarction. ICAM-1 and IL-6 plasma levels and/or function may be genetically influenced. We sought to evaluate distribution and reciprocal interaction of IL-6 G/C gene promoter polymorphism and ICAM-1 E/K gene polymorphism in Chinese patients with cerebral infarction .Methods: 112 cerebral infarction patients and 105 control subjects were recruited and the K469E polymorphism of ICAM-1 gene was analyzed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).The measurement data was analyzed statisticly by t-test and enumeration data by chi-squere test and Logistic regression analysis was used to control the confounding factor.Results: The average age of cerebral infarction group and control group (64.92±11.16 and 63.91±11.96 .respectively) is of no difference (P=0.523).The percentage of gender between two groups has no significant difference (x~2=0.52, P=0.472).The history of hypertension, diabetes, hyperlipodemia and smoking in cerebral infarction group are all higher than control group(P<0.05).KK and CC genetypes were significantly higher in cerebral infarction group compare with control group (OR. 0.343;95%CI, 0.195-0.604;P<0.001) (OR, 0.321;95%CI, 0.175-0.590;P<0.001) . KK/CC combined homozygosity have a further increased risk of cerebral infarction analysised by Logistic regression analysis (OR, 0.262;95%CI, 0.108-0.638;P=0.003) .Conclusions: There is a synergistic effect of IL-6 G/C and ICAM-1 E/K gene polymorphisms in patients with cerebral infarction. Reciprocal interactions between genotypes may contribute in determining the hereditary susceptibility for cerebral infarction.