The Research On Immune Gene Which Affect Deficiency Of Kidney-YANG Osteoarthritis

BackgroundAfter the Human Genome Project to be completed, the arrival of post genome era provides a new opportunity to study the Zheng of Traditional Chinese Medicine (TCM). At present, how to explore "Zheng" are the important focuses of combination of TCM with Western medicine research. Because of the complexity of Zheng, we used the method which link disease with Zheng. Microarray technology is a way of building bridges between disease and Zheng. It has positive meanings to clarify many problems in the current Zheng study.Objectiveâ‘  To study the differential expression genes of deficiency of kidney Yang â‘¡ To research differential expression immune gene of deficiency of kidney YangMethodsâ‘  Based NCBI database and GO database, taked the TCM Bianzhenglunzhi as the guiding ideology, refer to the modern medical model, combined the disease with Zheng (disease called by Western medicine and Zheng on TCM), we studied the microarray of patients who have osteoarthritis and deficiency of kidney Yang. We focused on the view of modem immunology, searched for relevant immunogenes, and the changes of immunogene in the patients who have deficiency of kidney Yang.â‘¡ We studied the topic on many subjects such as TCM, western medicine, molecular biology, biodemography, bioinformatics. We picked the samples (16 patients with osteoarthritis and the deficiency of kidney Yang, 8 norm person as the control group)from 2 areas, made 6 microarrays (4 single samples and 2 compond samples), and studied the differential gene microarray of the patients with deficiency of kidney Yang, search for relant immunogene.Resultsâ‘  We systematically analyzed 16 patients' Zheng scores who have deferency of kidney Yang and the control group's Zheng scores.â‘¡ We got 66 differential genes in six microarrays. Furthermore, after we put the 66 genes into network database, 61 genes can be identified in the network database. Among the 61genes, 22 genes are related to immune, which can tell us the close relationship between the deficiency of kidney Yang and immunogene expression.â‘¢ We analyse biological functions and pathways of the genes in 6 microarrays by using the network database.â‘£ We established the genetic basis of the combination of disease and Zheng,...