| Perimenopause and postmenopause women are trended to increase body mass and be obesity due to ovary function and the estrogen level decrease. At present, hormone replacement therapy is widely used in clinical application to treat perimenopausal disease, but it also could cause many side effects. The Radix Paeoniae Alba (RPA) is one of the Chinese herbal medicines frequently used in China. However, there are few reports about its effects on obesity in perimenopause and postmenopause women. The purpose of this experiment includes the following aspects: firstly to set up one of the animal models simulating perimenopause and postmenopause women's obesity by ablating the bilateral ovaries of the female rats, secondly to study the regularity of the energy metabolism in ovariectomized rats, thirdly to investigate the mechanism of the suppressive effects of RPA on obesity in ovariectomized rats.Healthy female SD rats with the body mass of 220.7±3.0g were chosen and divided into the following three groups at random: sham-operated group (SHAM, 8 rats), ovariectomized group (OVX, 8 rats) and Radix Paeoniae Alba group (OVX+RPA, 7 rats). Before operation, all rats had been fasted for 12 hours and the water was provides ad libitum. After having been narcotized by cloraminone(100mg/kg) intraperitoneal injection, the rats of OVX group and OVX+RPA group were underwent the bilateral ovariectomy, The rats of SHAM group were also operated, but their ovaries were not cut off. After the operation, the rats of each group ate and drank normally, but the rats of OVX+RPA group began to drink water extracts of RPA whose concentration increased gradually from 1% to 12% in five days and maintained 12% to the end of the fifty-fifth day while the food were not limited. It was the rules that the RPA concentration could not affect the normal food and water intake of the rats every day. The body mass were measured every five days and continued to do this to the end of the experiment. The quantity of food and water intake of each rat were noted daily and also lasted to the end of the experiment. At the end of the fifty-fifth day, after 12 hours fasting, the blood of the rats were collected from femoral artery to measure blood glucose and serum level of estradiol (E2), insulin, aspartate aminotransferase (AST), alanine minotransferase (ALT) , total cholesterol (TC), triglyeride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), total anti-oxidative capacity(T-AOC), malondialdehyde (MDA), leptin, interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α). The body length was measured and Lee's index was calculated exactly. All data were statistically analyzed by analysis of variance (ANOVA) (x±s). The main results were as follow:1. Compared with SHAM group, rats in OVX group had increased food intake , body mass, serum level of AST, ALT, HDL, LDL, TG, TC, MDA, leptin, TNF-αand decreased the serum level of E2.2. Compared with OVX group, the rats drinking the water extracts of RPA in OVX+RPA group could significantly restrain the augmentation of food intake, body mass, Lee's index and serum level of TG, MDA, leptin, TNF-α.These results indicate that the water extracts of RPA could inhibit the food intake of the ovariectomized rats, affect the serum level of the hormone relating obesity, and suppress the obesity of the rats induced by the ovariectomia. In addition, RPA could ameliorate lipid metabolic disturbance, eliminate the redundant free radical in vivo, and elevate anti-oxidative capacity in ovariectomized rats. |
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