The Study Of Expression Of RASSF1A And Survivin Genes In The Non-small Cell Lung Cancer And The Clinical Significance

Objectvie: To investigate the association between the expression of RASSF1A and Survivin proteins in non-small cell lung cancer (NSCLC) and their clinicopathological features and study the clinical significance, protein expression of RASSF1A and Survivin genes in NSCLC was detected by high throughput tissue microarrays technology combined with immunohistochemistry.Methods: (1) NSCLC tissue microarrays (TMA) including 95 samples was constructed. (2) Immunohistochemistry S-P was used to detect the expression of RASSF1A and Survivin proteins in the NSCLC tissue microarrays containing of 79 cases of NSCLC and 14 normal epithelial tissues from non-tumor lung tissue. (3) Meanwhile, we analyzed the association between expression of RASSF1A and Survivin protein and the clinicopathological features, also, the correlation between RASSF1A and Survivin protein in the NSCLC.Results (1) NSCLC TMA including 95 samples was constructed successfully. (2) The positive expression of RASSF 1A in NSCLC (46.8%, 37/79) was significantly lower than that of in non-tumor lung tissues (92.9%,13/14) (P＜0.001)。(3) The percentage of RASSF1A protein expression in the stageⅠand stageⅡof NSCLC was evidently higher than that of in the stageⅢ(P＜0.0001, respectively)。The percentage of RASSF1A in NSCLC with lymph node metastasis was observably lower than that of in cases without lymph node metastasis (P＜0.05), while the expression of RASSF1A had no significant correlation with sex, age, tumor site, tumor size, histologicaltype and tumor grade of NSCLC (P＞0.05). (4) The positive expression of Survivin in NSCLC (78.5%, 62/79) was significantly higher than that of in non-tumor lung tissues (0%,0/14) (P＜0.0001)。The percentage of Survivin protein expression in the stageⅠand stageⅡof NSCLC was significantly lower than that of in the stageⅢ(P=0.003, P=0.001), but the expression of Survivin had no evident association with sex, age, tumor size, tumor site, histologicaltype, tumor grade and lymph node metastasis of NSCLC (P＞0.05). (6) Furthermore, there was observably negative correlation between expression of RASSF1A protein and that of Survivin protein in NSCLC (r=-0.780; P＜0.0001).Conclusions: (1) NSCLC TMA including 95 samples was constructed successfully. (2) The loss expression of RASSF1A protein in NSCLC was related to its lymph node metastasis and TNM stage. The results suggested that loss of RASSF1A protein might improve the development and progression of NSCLC, which might be acted as one helpful molecular marker to predict the prognosis of NSCLC. (3) The over expression of Survivin in NSCLC was positively related to its TNM stage, the result revealed that Survivin protein might involve in the development and progression of NSCLC, and indicate that patients with positive expression of Survivin protein might have poor prognosis. (4) Negative correlation between expression of RASSF 1A protein and that of Survivin protein in NSCLC might demonstrate that disturbed balance expression between RASSF1A and Survivin proteins might play important roles in the development and progression of NSCLC.