Studies Of Effect Of QizhiWeitong Granule On Gastrointestinal Motility In Mice

Qizhiweitong Granule (QZ), which is composed of Bupleurum, fructus aurantii, Glycyrrhizae, Paeonia lactiflora pallas, corydalis, is a representative prescription being used to treat feeling of fullness in the chest and upper abdomen. It is reported that QZ has broad pharmacological effects, such as antiulcer, against inflammation, analgesic, etc. QZ is a traditional Chinese herb which was used in treatment of many gastrointestinal diseases. The present study was designated to study the regulatory effects of QZ on gastrointestinal function, and to compare the efficacy of QZ and its new formula QZ1, QZ2 and commercial drugs in mice.1. Firstly, the effects of QZ on the gastrointestinal function of mice was studied. The results showed that(1) QZ (0.975, 2.925, 8.775g/kg, 6d, p.o.) has the inhibitive effect on gastric emptying in normal mice, but had no effect on intestinal propulsive movement. The value of ED₅₀ on gastric emptying was 0.803 g/kg (5d, p.o.).(2) QZ significantly antagonized the gastric emptying inhibition induced by atropine, dopamine or morphine in mice, QZ also antagonized neostigmine-induced gastric emptying stimulation in mice. Moreover, QZ markedly antagonized the intestinal propulsive inhibition induced by morphine or atropine in mice, but had no effects on the intestinal propulsive inhibition induced by dopamine. It had no significant effect on neostigmine-induced intestine propagation stimulation in mice.(3) QZ markedly antagonized the gastric emptying inhibition induced by diabetic gastroparesis in mice, but had no effects on the intestinal propulsive inhibition induced by diabetic gastroparesis.(4) QZ significantly increased the incubation period of hot-plate reaction and showed significantly suppressive effects on writhing induced by acetic acid.2. Comparative studies of QZ and QZ1 or QZ2 on gastrointestinal motility effect showed that(1) QZ1 possesses analgesic and anti-inflammatory effects. (2) QZ1 (9.75g/kg, p.o.) has the inhibitive effect on gastrointestinal motility in normal mice. The value of ED₅₀ was 2.26 g/kg (5d, p.o.).(3) QZ1 and QZ2 had no effect on gastrointestinal motility inhibition induced by atropine, dopamine or morphine in mice.3. The studies comparing the differet activities of QZ and related commercial drugs on gastrointestinal motility in mice showed that(1) Commercial drugs (Duopanlitong pian, Wei U Dianqie Lu Jiaonangâ…¡, Kuihua Weikangling Jiaonang) had no dual-directional regulating effects on gastrointestinal motility.(2) It also showed no significant effect on gastrointestinal motility inhibition induced by diabetic gastroparesis in mice.In conclusion, the present study firstly showed the significant dual-directional regulatory effect of QZ on gastrointestinal motility in mice and can improve the symptoms diabetic gastroparesis. QZ and QZ1 possess analgesic and anti-inflammatory effects. The results of gastrointestinal transit and diabetic gastroparesis experiments suggested that QZ may be superior to commercial drugs. The study of this paper establishes the pharmacology foundation of further development of QZ and implied a potential new indications in clinical applications.