Experiment Study Of CD40 Ligand To Treat Breast Cancer

Breast caccer is one of the most common carcinoma of women.The over view to breast cancer has been changed: as present,we think breast cancer is disease all over the body at first,the mass of breast is the concertration of the disease.So the treatment to breast cancer is first focus on the systemic treat.Athough we have used chemotherapy, ratiotherapy end endocrinotherapy, there is no obviously improved survival. We should look for more effective treatment. It is essential for emerge,developing and metaptosis of breast cancer of new angiopoiesis through the developing of iatrology.Recently it is warm spots to treat new vessel of breast cancer. Therefore we design the following two experiment.PartⅠInvestigation of inhibit effect of CD40 ligand on angiopoiesisObjective:To establish chicken chorioallantoic membrane model,discuss the inhibting effect on angiopoiesis.Methods:Get 30 hatching chick embryo of 8 days, divide into 3 groups in random.,10 every group. Unfolding air chamber sterile,disclose chick embryo endomembrane,add PBS ( negative control ) ,CD40 ligand(200ug/ml),Genistein(positive control,100umol/l) to allantocherion ofchick embryo., close tightly gap using Co60 adhesive tape.after hatching 10 days,open air chamber, mounting allantocherion 5~10 min using dimethyl ketone and dehydrated alcohol.cut allantocherion covering filter paper, categorize vessel. Statistics all vessel,every size of sample is 1.5cm.×1.5cm.Results:CD40 ligand and Genistein inhit significantly angiopoiesis, of the total inhibiting second class and third class vessel is significant.Conclusions:CD40 ligand can inhibit vascular endothelial cell.PartПExperiment study of the the effect of CD40 ligand on nude mouseObjective:In this study, we aimed to investigate the inhibit effect and mechanism of breast cancer cells through CD40 ligand and the inhibit effect and mechanism of breast cancer new vessels.Methods:We generated mammary cancer animal models by MDA-MB-231 cell line of expressing highly CD40.After animal models grouth to 1.5~2.0cm ,The models was randomly into four groups . Every group was treated once per week for 3weeks by tumor local injection of PBS,ADM(5mg/kg),CD40ligand (200ug/ml) and combination. In the fourth week, athymic mouse was sacrificed and subcutaneous tumor was selected under sterile conditions. Weight and volume of body of tumor were measured and moreover ratio oftumor growth inhibition was analyzed. The morphological changes or ultramicro-structure changes of apoptotic tumor cells was observed by TUNEL.Caspase3, as apoptosis-associated protein, was tested by immunohistochemistry. Mcrovessel density of tumor was calculated by blood vessel endothelium staining with CD105.Results: After animal model treating for 3 weeks, the resuls showed that tumor weight, tumor volume and tumor growth inhibition rate in ADM group,CD40 ligand group and combination group were significantly different from that of PBS group(P<0.05), ADM group and CD40 ligand group had no difference compared with each other(P>0.05). However, ADM group,CD40 ligand group was not as good as that of combination group; There were significant difference in three items between combination group and PBS group. The volume diminution of cancer cells, karyopycnosis, karyorrhexis and apoptotic body can be observed in those three groups by TUNEL .Their apoptotic rates differed from PBS group significantly(P<0.05), so did the expression of Caspaase3 protein. According tumor MVD marked by CD105, ADM group,CD40 ligand group and combination group can inhibit neovascularization obviously, which existed notable disparation compared with PBS group(P<0.05). Nevertheless, Apparent difference was shown between ADM group,CD40 ligand group and vacant combination group.Conclusions:CD40 ligand combining antitumor drug can treat breast cancer.