Evaluation Of Rat Model With Syndrome Of Accumulation Of Cold Fluid In The Lung In Chronic Obstructive Pulmonary Disease

Objective: This thesis expounds systematically the theoretic problems about the syndrome of accumulation of cold fluid in the lung in chronic obstructive pulmonary disease(COPD) to enrich the theory of the syndrome. The rat model with the syndrome of accumulation of cold fluid in the lung in COPD was established by inhalation cigarette,exposure to cold and intratracheal instillation of lipopolysaccharide(LPS) ,to evaluate the credibility of rat models and observe the efforts of stomach irritation with"Yang-Warming and Fluid-Dispersing Formula"and inhaled Budesonide on the model.Methods: This research based on the viewpoints of two theories, namely, the combination of exopathogens and inpathogens, coldness of body and drinking cold tends to impair lung, combining with the nosogenesis of COPD. Pathologic rat models with the syndrome of accumulation of cold fluid in the lung in COPD were set up with the methods of feeding with ice water with cold property in the cold environment of winter,. inhalation cigarette and intratracheal instillation of LPS.The spontaneous cure of rat models were observed.And the models were treated with"Yang-Warming and Fluid-Dispersing Formula"and Budesonide. Then observe the relevant indexes including symptoms and signs, pathological section of lung tissues, interleukin-8(IL-8) and interleukin-10(IL-10) in blood serum, and also the lung function measurements were carried out.Results: The symptoms and signs of pathologic rat models with the syndrome of accumulation of cold fluid in the lung in COPD were in accordance with clinical symptoms and signs of patients of the syndrome of accumulation of cold fluid in the lung. The changes of microcosmic indexes(except IL-8 and IL-10) coincided with those of COPD related to the syndrome. For example, the expressive index of avoirdupois were alleviated, dynamic lung compliance(Cldyn) decreased while inspiratory resistance(Ri) and expiratory resistance(Re) increased, lung tissues were inflammatorily damaged,IL-10 and IL-8 had no significant changes(P>0.05) comparing with the control group's. And there were no obvious changes after 4 weeks. The two remedy in the treated group ameliorated above indexes except IL-10 and IL-8 in blood serum.Conclusion: It is feasible to set up the rat models with the syndrome of accumulation of cold fluid in the lung in COPD using those methods of experiment. The symptoms and signs, lung function and inflammatory reaction in airway of pathologic rat models could be ameliorated well by"Yang-Warming and Fluid-Dispersing Formula"and Budesonide.,but there was no significant differences in the therapeutic effect of two drugs.