Correlation Of Eukaryotic Initiation Factor 4E And Osteopontin Of Serum And Tissue In Patients With Ovarian Cancer

Objective: Ovarian tumour is a common gynaecological tumour. Ovarian cancer is one of the three main gynaecological malignancies, there are no effective methods in clinic for early diagnosis. The five-year survival rate is about 25%～30%. Ovarian cancer becomes the highest mortality tumour for the well developed of diagnosis and therapy of cervical carcinoma and endometrium carcinoma. People make great efforts to search for the special biomarker of ovarian carcinoma for early diagnosis, monitorring disease, observation of curative effect and predicting prognosis. We investigatted the correlation between eIF-4E,OPN and ovarian cancer by detecting the expression of eIF-4E,OPN in ovarian cancer tissue and their serum concentration to reveal the pathogenesis of ovarian cancer on genetic level. We Explorried the possibility of eIF-4E and OPN as tumour markers combined CA125 for early diagnosis, detecting curative effect and monitoring recurrence.Methods: select 46 cases of ovarian epithelial carcinoma, 16 cases of borderlin ovarian epithelial tumour, 12cases of benign ovarian epithelial tumour, 12 cases of normal ovarian tissue, all confirmed by pathology. 1. Detecting the expression of eIF-4E, OPN in the above mentioned tissues by immunohistochemistry, measurring the value of absorbency (A) by pathological image analysis software of medical image analysis system, calculating average value of absorbency to make semiquantitative analysis. 2. Detecting the serum concertration of eIF-4E, OPN in the above samples, all serum concertration of CA125 are detecting by microsome chemiluminescence method in laboratory of our hospital. 3. Statistical method: all statistical analyses were performed with spss13.0 statistical software package, measurement data was expressed as mean SD values. The enumeration data were compared with the chi-square test. The positive expression rate in different pathological types, clinical staging and pathological grade were compared with the chi-square test and the fisher's exact probability test. The comparison of the expression intensity in tissues was used Wilcoxon signed-rank test. The comparison of multiple mean was used analysis of variance after the equal check of variance, and the two-two comparison among the means was done by LSD-t method. The correlation between serum concentration and tissue A value was evaluated by spearman correlation analysis. The level of significance was set at P<0.05.Results: 1 The expression of eIF-4E and OPN in four different tumor tissues: the eIF-4E protein was mainly expressed in plasma of ovarian tumor epithelial cells, whereas it was in cytoplasm for OPN. In normal ovary, benign, boderline and maligant ovarian lesions , the positive expression rate of eIF-4E protein was 8.3%(1/12), 16.7%(2/12), 56.3%(9/16)and 84.8(39/46), respectively; it was 16.7%(2/12), 16.7%(2/12), 62.5%(10/16) and 89.1%(41/46)for OPN protein. The positive expression rate of eIF-4E protein in normal ovary and benign ovarian lesions was lower than that in boderline and maligant ovarian lesions, the difference was remarkable between the former two and the latter two(P<0.05), but there were no significant difference in former two and in latter two(P>0.05);For OPN protein , the comparison between the former two and the latter two was the same as eIF-4E protein, but there were no significant difference in former two and in latter two(P>0.05).2 The expression of eIF-4E and OPN in ovarian carcinoma tissues: the positive expression rate of eIF-4E and OPN protein in ovarian serous cystadenocarcinoma was higher than that in mucous cystadenocarcinoma and endometrioid adenocarcinoma, but there were no significant difference among the three groups(P>0.05). comparing the positive expression rate of eIF-4E and OPN protein in clinical stage and histopathologic grades , there were no significant difference(P>0.05); comparing the intensity of their expression, also no significant difference(P>0.05), but there was the ascending trend.3 The A value of eIF-4E and OPN protein in ovarian carcinoma: With elevating clinical stage, the A value of eIF-4E and OPN protein were increased, in stageⅢ,Ⅳ, it was significantly higher than that in stageⅠ,Ⅱ, the difference was remarkable among the four stages(P<0.05). The A value of eIF-4E and OPN protein in G2, G3 grade was significantly higher than that in G1 grade. The difference was remarkable in the former two and the latter (P<0.05), resepectivly, there were no significant difference in former two(P>0.05).4 The concentration of serum of eIF-4E, OPN and CA125: in boderline and maligant ovarian tumour patients, the concentration of serum of eIF-4E, OPN and CA125 were significantly higher than that in normal ovary and benign ovarian tumour patients(P<0.05), there were no significant difference between the former two and the latter two(P>0.05). 5 The serum concentration of eIF-4E, OPN and CA125 in different histological types: in serous cystadenocarcinoma, the serum concentration of CA125 was higher than that in mucous cystadenocarcinoma and endometrioid adenocar- cinoma, (P<0.05). Comparing the serum concentration of eIF-4E and OPN in three histological types, there were no significant difference(P>0.05), but the trend was ascending.6 The serum concentration of eIF-4E, OPN and CA125 in different clinical stages: the serum concentration of eIF-4E, OPN and CA125 inⅢ,Ⅳstage was significantly higher than that inⅠ,Ⅱstage(P<0.05).7 The serum positive expression rate of eIF-4E, OPN alone or combined with CA125 on the detection of ovarian carcinoma: eIF-4E or OPN combined with CA125 and using three of them, the positive expression rate in ovarian carcinoma were higher than that alone(P<0.05).8 The ralation between the expression of eIF-4E and OPN of serum and tissue in patients with ovarian carcinoma : the expression of them was positively correlated, the correlation coefficient was 0.45, 0.48, respectively(P<0.05).Conclusions: The positive expression rate of tissue and serum concentration of eIF-4E and OPN protein in boderline and maligant ovarian lesions are higher than that in normal ovary and benign ovarian lesions, with elevating clinical stage, the expression of their protein are increased, the level inⅢ,Ⅳstage is higher than that inⅠ,Ⅱstage. The expression of eIF-4E and OPN in ovarian carcinoma tissues were correlated with their concentration in serum. We detected the eIF-4E, OPN combined with CA125 in serum of ovarian tumour patient could improved the positive expression rate in ovarian carcinoma diagnosis. So, eIF-4E and OPN gene takes part in the development of malignant epithelial ovarian tumors. They may be regarded as the important biomarkers for early diagnosis, they can evaluate the curative effect of ovarian cancinoma and they may be become the new target for the therapy of ovarian carcinoma .