Effect Of Iron And Iron Complex In Inhibiting Psoriasis

ObjectiveThe effect of iron and iron complex on inhibiting psoriasis development in mouse vaginal epithelium model and mouse tail scale epidermis model were explored.Methods1.Effects of iron and iron complex on the vaginal epithelium mitosis of estrone periodical mice model.Female kunming mice were used to establish the epidermis hyperplasia of estrone periodical mice model.They were randomly divided into nine groups: negative control,positive control,low,middle,high dosage of FeCl₃,low,middle, high dosage of Fe-EDTA and iron lactate.The mice were sacrificed by cervical dislocation after the last administration,and the vaginal mitosis index was observed under optical microscope.2.Effects on mouse mouse-tail scale epidermis modelsMice used as the epidermis parakeratosis model were randomly divided into nine groups indicated as above.The formation of mouse-tail scale epidermis was observed and the numbers of scale with granular layer per 100 scales were examined by optical microscope.3.Analysis of essential elements.To investigate concentrations of copper,iron,zinc,calcium,magnesium, manganum and selenium,liver,spleen,kidney and tail of mice in all groups were taken and digested to analyze the elements concentration by atomic absorption spectrophotometry.4.Effects on recovering the anti-oxidizing systemThe activities of SOD,GSH-PX and the content of MDA in the homogenate of liver,kidney and spleen of the mice were determined in each group.5.Effects on the serum level of IL-2 and IL-10Radioimmunoassay was applied to measure the serum level of IL-2 and IL-10 in each group.Results 1.Effects of zinc and zinc complex on mouse vaginal epithelium modelEvery iron spiked groups as well as methotrexate group could obviously inhibit the mitosis of vaginal epithelial cells(P＜0.01).No statistical significance was found between each iron spiked group and the positive control group(P＞0.05).2.Effects on mouse tail scale epidermis modelsThere was statistical significance between each iron spiked group as well as the positive control and the negative control group(P＞0.05).3.Effects on removing other essential elementsIron contents of each iron spiked group and methotrexate group in spleen were higher than that of the negative control group(P＜0.05).No statistical significance was found between the iron spiked group and methotrexate group(P＞0.05).In comparison with iron contents,no statistical significance existed among the groups in the liver, kidney and tail(P＞0.05).In comparison with copper,zinc,magnesium,manganum and calcium contents, no statistical significance existed among the groups in the liver,spleen,kidney and tail(P＞0.05).4.Effects on recovering the anti-oxidizing systemIn comparison with the corresponding data of the negative control group,the activities of SOD decreased while the contents of MDA increased.However,no statistical significance existed in the livers,kidneys and spleen.5.Effects on the serum level of IL-2 and IL-10The serum level of IL-2 in each iron spiked group as well as methotrexate group was higher than that in the negative control group,but no statistical significance existed(P＞0.05).The serum level of IL-10 increased in each iron spiked group,but there was no statistical significance(P＞0.05).Conclusion1.As a result of iron replenishment,the mitosis of vaginal epithelial cells was obviously·inhibited,and the formation of granular layer in mice tail scale was significantly promoted.2.Iron replenishment could obviously enhance iron contents in spleen and had no obvious effect on other essential elements such as copper,zinc,magnesium, manganum,and calcium.3.It could also enhance the activity of the antioxidant enzyme.4.There were no obvious effects on the serum level of IL-2 and IL-10.