Experimental Research About Substance Basis And Mechanism Of Xinwei Decoction In Overall Modulating For Treating Functional Dyspepsia (FD)

Objective:The main objective of this research is:l To attempt to make FD animal model with "irregular food intake match with clamping the tail and stimulation method".2 To research the Substance Basis and Mechanism of xinwei decoction in overall modulating for treating FD,discussing the scientific evidence of it in treating FD.Methods:The 1st step:To make the model and therapy:40 health apinoid male Wistar rats,mean weight of(200±20)g, were divided into 4 groups(10 of one group),Selected 1 group as blank contrast group(BG),fed by normal ways for 14 days.Other 3 groups were made models by "irregular food intake match with clamping the tail and stimulation method" for 14 days.Then filled the BG rats with normal saline by intragastric administration(10ml/kg) for 14 days;and named other 3 groups as: model contrast group(MG),xinwei decoction group(SG) and Domperidone group(DG),filled respectively with normal saline, xinwei decoction and Domperidone liquor by intragastric administration for 14 days.Observed the change of the general condition and the amount of food and drink. The 2nd step:To detect the indicatrixes:Experiment of stomach emptying:Left the 4 groups during 24 hours without feeding and drinking.Then fill them with amylum pasta(1g/ml) by intragastric administration(10ml/kg).After 1 hours standing,killed them by decapitation,gathered 5ml of blood, put into the anticoagulation tube and placed in the refrigerator, waited for detecting NO andMTL.Then cut the belly open,took out of the stomach and weighted it.Then cut the stomach open,washouted the content,weighted the empty stomach.Calculated the empty rate, and observed the stomachmucosa.Cut the sinus ventriculi and put it into formaldehyde liquor of 10%for fixation,the remanent stomach tissue were reserved and levigated into homogenate of 10%waited for detecting NO.The NO of the stomach tissue and blood serum had been detected by "nitrate reductase method";the MTL of the blood plasma had been detected by "radioimmunoassay method"To observe the constitution of the sinus ventriculi tissue:Take out the sinus ventriculi(reserved at the experiment of stomach emptying) from the formaldehyde liquor,dewatered by gradient alcohol,made clearing by dimethyl benzene,embeded by ozocerite, made microtome section in 4μm' s thickness,stained by hematoxylin and eosin,observed the constitution of the sinus ventriculi tissue by the light microscope.Results:1,The general condition and stomach mucosaBG rats had steady amount of food and drink;significant increasing of the weight;pure white and soft capill;tectonic dejecta without thick smell;gentle temper and good mental status;erythroic colored and unabroken stomach mucosa.MG rats had indiscriminate amount of food and drink;yellow and dry capill;deformed and foul dejecta;crabby temper and bad mental status;congested and edematus stomachmucosawithout impairment. DG and three Chinese herbal groups were as same as MG before treatment;while after treatment,improvement had taken place about the amount of food and drink,weight,capill color and dejecta;with the gentler temper,better mental status and erythroic colored and unabroken stomach mucosa.2,Compared about the stomach empty rate(%)Compared with MG(16.22±3.62),ZG(28.19±1.65) could significant increase the stomach empty rate(P <0.05);ZG was significant better than DG(22.69±1.96)(P(0.01).3,Compared about the NO of stomach tissue and blood serumStomach tissue(μmol/gprot):Compared with MG(4.18±0.12), ZG(2.80±0.23) could significant decrease the NO(P<0.01),and were significant better than DG(3.76±0.21)(P<0.01)。Blood serum(μmol/L):Compared with MG(59.75±10.86), XG(42.46±4.54) could significant decrease the NO(P<0.05),and were equipotent with DG(47.51±9.59)(P>0.05).4,Compared about the MTL of the blood plasma(pg/ml)Compared with MG(385.21±10.98),ZG(430.03±8.23) could significant increase the MTL(P<0.01);ZG were significant better than DG(408.28±11.10)(P<0.01).5,The constitution of the sinus ventriculi tissueMG rats' mucosas of sinus ventriculi were unabroken,with congestion,edema and infiltrating of inflammatory ceils.DG and Chinese herbal groups' mucosas were unabroken,without such appearance above-mentioned.Conclusion:1,Xinwei decoction in overall modulating for Treating FD for it can improve FD rat' s general condition and stomach mucosa appearance;increase it' s stomach empty rate;lessen the NO' s inhibition of stomach empty;increase the MTL to promote the stomach empty.1.This experiment confirmed that the xinwei decoction can directly promote gastrointestinal motility(improve gastric emptying rate),and can change the level of gastrointestinal hormones, Combination of that party has shown accompanied by depression and anxiety on the status of functional dyspepsia has obvious therapeutic effect[6]Clinical studies;Therefore,the xinwei decoction General power of western medicine,gastrointestinal medicine,antidepressants and adjusting the characteristics of gastrointestinal hormones,Embodies the whole concept of Chinese medicine treatment of disease characteristics.