The Expression Of CD147 And MMP-2 In Cervical Cancer And Associated Research

Objective Cervical cancer is a common malignancy among women,ranked second among in the world of women malignant tumors.Extracellulr matrix metalloproteinase inducer(CD147/EMMPRIN) is one of the molecules involved in regulating the expression of matrix metalloproteinases(MMPs).The goal of this study was to analyze the expression levels of CD 147 and MMP-2 in normal cervical tissue,cervical intraepithelial neoplasia(CIN) and cervical carcinoma,and to analyze its relationship with clinicopathological parameters of cervical cancer-related links to explore the above-mentioned target happened in cervical cancer development, invasion and metastasis,for the diagnosis and treatment of cervical cancer to provide a new and effective target.Methods Using streptavidin-biotin-protein - linked peroxidase method(ie,SP) immunohistochemical method to detec the expression of CD 147 and MMP-2 in 20 cases of normal cervical tissue,21 cases of CIN tissue,59 cases of cervical cancer. Analysis of cervical cancer in CD 147 and MMP-2 expression and clinical stage, histological grade and other clinicopathological parameters of relevance,at the same time the relationship between CD 147 and MMP-2 was studyed in the development of cervical cancer for a preliminary study.Results1.CD 147 and MMP-2 were expressed mainly in cancerous lesions,but also expressed in some normal tissues.2.Positive expression rate of CD 147 in normal cervical tissues,CIN,cervical cancer tissues were 30.0%,81.0%,91.5%,the trend has gradually increased(P＜0.05). Among them,significant difference was existed between normal cervical group and CIN group(P＜0.05),also between normal cervical group and cancer group(P＜0.05), CIN group and cancer group(P＜0.05).3.Positive expression rate of MMP-2 in normal cervical tissues,CIN,cervical cancer tissues were 15.0%,38.1%,80.0%,the trend has gradually increased(P＜0.05). Among them,significant difference was existed between normal cervical group and cancer group(P＜0.05),also between CIN group and cancer group(P＜0.05).There was no significant difference between normal cervical group and CIN group (P＞0.05). 4.In cervical carcinoma,positive rate of CD 147 protein expression with lymph node metastasis group was higher than that without lymph node metastasis group (x~2=4.138,P＜0.05);Expression of CD147 in cervical cancer were not related with age,stage,gross type,the degree of differentiation,histological types(P＞0.05).5.In cervical cancer,positive rate of MMP-2 protein's expression at FIGO stagesⅠ-Ⅱa period was higher than theⅡb-Ⅳperiod(x~2=9.423,P＜0.05),there is lymph node metastasis group was higher than that without lymph node metastasis group(x~2=5.019,P＜0.05);Expression of CD147 in cervical cancer were not related with age,gross type,the degree of differentiation,histological types(P＞0.05).6.The relationship between CD 147 and MMP-2 was positively correlated,and the correlation coefficient rs = 0.283(P＜0.05).Conclusion CD 147 and MMP-2 in the normal cervix,CIN,cervical carcinoma was higher trend;CD 147 in lymph node metastasis is higher than without lymph node metastasis.MMP-2 in the phaseⅡb-Ⅳ,there is lymph node metastasis of cases of cervical cancer is higher than theⅠ-Ⅱa period,no lymph node metastasis;There is synergy between CD 147 and MMP-2;CD 147 and MMP-2 plays an important role in cervical cancer invasion and metastasis,Combined detection of CD 147 and MMP-2 may be a new target of cancer diagnosis and treatment and a new way to assess the severity of cervical cancer.