Antiangiogenesis Effect Of Celecoxib On Xenografted Human Osteosarcomar Stem-like Cells In Nude Mice

Objective : To explore the effect of Celecoxib on growth and angiogenesis of the xenografted human osteosarcoma stem-like cells in nude mice.Method: Human osteosarcoma stem-like cell ("sarcospheres") were isolated from human osteosarcoma cell line MG-63 with serum-free culturing techniques. The xenografted models of human osteosarcoma stem-like cells were successfully established using subcutaneous injection of human osteosarcoma stem-like cell in 30 nude mice and randomly divided into the control and Celecoxib groups. Oral administration was carried out in both groups , with 25 mg/(kg·d) of Celecoxib in the Celecoxib group and the same volume of deionized water in the controls. This treatment last for 15 days and all mice were sacrificed after 27 days to investigate the tumor volumes, tumor inhibitory rate. The VEGF expression and CD₃₄ tagged MVD value was detected by immunohistochemical staining.Result: The isolated human osteosarcoma stem-like cell exhibit tumorigenesis and the animal model was established, after 15 days of administration: the tumor inhibitory rate was 23.2%, and the tumor volumes, VEGF expression and MVD value were significantly lower than those in control group.Conclusion: Human osteosarcoma stem-like cells could be establish xenografted osteosarcoma in nude mice. Celecoxib showed a significantly inhibitive effect on growth, VEGF expression and angiogenesis of xenografted osteosarcoma.