| Immune infertility accounts for about 10% to 30% in female infertility, can be divided into Alloimmune infertility and Autoimmune infertility. Alloimmune infertility is caused by the AsAb, while Autoimmune infertility is mainly caused by anti-endometrial antibody and zona pellucida antibodies in serum of Infertility. Currently, western medicine mainly help conception through steroids, condom therapy, artificial insemination and in vitro fertilization method and embryo transplant, but the effect is not satisfied and it has the shortcomings of side effects and high cost. In recent years, traditional Chinese medicine in the treatment of immune infertility often makes satisfactory effect, and the majority of patients receive the recognition because of the low side effects and less cost. Many studies show that traditional Chinese medicine for immunological infertility have a better clinical effect, also the effect of acupuncture on the human body immune function is good.But the report which combines acupuncture and traditional Chinese medicine to treat the feminine immune infertility is very few, and the effect mechanism of combined therapy is not very clear. And for that Chinese medicine in immune infertility treatment superiority will be impeded.Objective:This research starts from the perspective of neuromodulation. With clinical observation and animal experiments, we adopt acupuncture with herbal treatment or herbel treatment for patients. After treatment, we observe the clinical curative effect and part of the mechanism on immune infertility by acupuncture.Method: 1. Clinical research. This study divide the patients who accord with the acceptable standards, in accordance with the principle of randomized controlled, into'Acupuncture with Herbal treatment'treatment group with 30 and'Traditional Chinese medicine'control group with30. Both two groups take traditional Chinese medicine'Guishen liquid'(Ripe rehmannia glutinosa 30g, dodder 30g,Ligustrum lucidum 15g,Chinese yam 15g, Medlar 20g,Shou Wu 20g, loranthus parasiticus 20g, dogberry 10g, herba epimedii 10g;add-subtract: partial blood stasis, add panax notoginseng 15g, Salvia miltiorrhiza 30g;if partial deficiency, add Codonopsis 15g, Astragalus membranaceus 30g;genital tract inflammation, add dandelion 30g), once a day, decoction, drink it in the morning and night, three months per course, a total of 1-2 courses. The treatment group also take acupuncture, the main points are Shenshu, Sanyinjiao, Taixi, add-subtract:blood stasis, add Geshu;if partial deficiency, add Zusanli, Guanyuan;genital tract inflammation, add Fenglong. Once every other day,30 minutes keeping every time, three months per course, a total of 1～2 courses.2. Animal experiments.55 female rats are randomly divided into 5 groups, healthy control group8, positive control group 8, traditional Chinese medicine group 13, acupuncture group 13, acupuncture with herbal group13. Injecting lml antigen in some points in bilateral inguinal and neck back of each SD rat as the first immunization except the healthy control group. AsAb who has not been created in the first immunization should be intensified in the same way in next week. At the same time, injecting lml NS in some points in bilateral inguinal and neck back of the healthy control group, 1ml per each rat.After the modeling, we will start to give rats medicine for 4 weeks. Traditional Chinese medicine group, Acupuncture with herbal group are fed 4ml Guishen liquid daily, the healthy control group are fed 4ml NS daily. Acupuncture group, Acupuncture with Chinese medicine group start acupuncture. The rats are fixed on the rat trap, acupuncture Shenshu, Sanyinjiao, Taixi,30 minutes keeping every time,1 time per day for 4 weeks.Results:Clinical research:In the treatment group,the total clinical effects is 93.33%, the negative rate of ASAb is 56.67%, and the pregnancy rate is 36.67%. In the control group, the total clinical effects is 86.67%, the negative rate of ASAb is 30%,and the pregnancy rate is 13.33%. and the levels of serum anti-sperm antibodies (ASAb) in the two groups after treatment has decreased. All are significant. Animal experiments:After treatment, the compares of serumβ-EP between Acupuncture with herbal group, Chinese medicine group and positive control group are significant, the compare of serumβ-EP between Acupuncture and Chinese medicine group and Chinese medicine group is also significant.Conclusion:1. This study adopts ELISA method for detection of serum ASAb. Using laboratory diagnosis immunity infertility index, with western medicine diagnosis technology,We overcome the shortage of Chinese medicine diagnosis of immune infertility.2. After treatment, combining modern medical diagnosis, we detect the level of serum ASAb and observe whether patients are pregnant. We find that the treatment of immune infertility in treatment group is effective, it can promote pregnancy.3. Acupuncture and Chinese medicine can adjustβ-EP, thereby adjust reproductive immune function by NIM, inhibit the generation of ASAb to improve patient reproductive immune environment.4. Combined treatment of Acupuncture and Chinese medicine is better than simply Chinese medicine and Western medicine treatment. It is worth to generalize and apply as treatment method.
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