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Effects And Comparison Of Olmesartan Medoxomil And Candesartan Cilexetil On Inflammation In Atherosclerosis Of Rats

Posted on:2012-07-10Degree:MasterType:Thesis
Country:ChinaCandidate:L WuFull Text:PDF
GTID:2154330332496450Subject:Cardiovascular medicine
Abstract/Summary:Request the full-text of this thesis
Objective:To study the effect of olmesartan medoxomil on inflammation in atherosclerosis(AS)rats and the comparision with candesartan cilexetil.Method: forty male Wistar rats were randomly divided into the following four groups: A=controlgroup, B=AS model group,C=candesartan cilexetil-treated group, D=olmesartan medoxomil-treatedgroup, there were 10 rats in each group. B,C and D groups reproduced AS model,C and D groupwhile giving candesartan cilexetil and olmesartan medoxomil to intervene respectively, the ratswere fed 8 weeks in total. At the beginning of the experiment and at the end of 8 week, body weightwere measured, lipid levels in plasma (TG, TC, HDL-C, LDL-C) were detected by chemicalmethod,the levels of serum MCP-1 and soluble P-selectin (sP-selectin) were measured byenzyme-linked immunosorbent assay (ELISA); At 8 week, to separate the thoracic aorta andabdominal aorta of rats quickly, the expression of CD40 of aorta in every group was detected byreverse transcript polymerse chain reaction ( RT-PCR) ,and finally to take pictures and observe withUV detector, then conclude the relative expression level of CD40 and CD40L in aortic tissue andcompare with each group.Results:①At 0 week,body weight and the lipid levels in plasma were no significant difference inall experiment group rats (P> 0.05); At 8 week, body weight and the lipid levels in plasma (TG, TC,LDL-C) of AS rats were significantly higher than A group (P<0.01), two drud-treated groups hadno obciously change to AS rats(P>0.05).②At 0 week, the levels of serum MCP-1 and sP-selectinshowed no significant difference in each group (P>0.05); At 8 week, the serum MCP-1 andsP-selectin contents of AS rats were obviously higher than A group (P<0.01), C group and D groupreduced the levels of serum MCP-1 and sP-selectin of AS rats significantly (P<0.01), whilecompared with C group, the serum MCP-1 and sP-selectin contents decreased in D group, there wasstatistical significance (P (P<0.05).③The expression of CD40 and CD40L in aortic structure ofAS rats was higer than A group (P<0.01), to compare with B group, the expression of CD40 andCD40L in C and D group decreased obviously(P<0.01), furthermore the expression of CD40 andCD40L in D group was lower than C group and the difference was statistically significant (P <0.01).Conclusions: Olmesartan medoxomil and candesartan cilexetil had no significant effects to ASrats on body weight and lipid levels in plasma , olmesartan medoxomil and candesartan cilexetilcould reduce the levels of serum MCP-1 and sP-selection, and decrease the expression of CD40and CD40L in aortic structure of AS rats significantly, besides the effect of olmesartan medoxomil was stronger than candesartan cilexetil, olmesartan medoxomil maybe play its anti-atherosclerosisprocess via anti- inflammatory effects.
Keywords/Search Tags:atherosclerosis, olmesartan medoxomil, candesartan cilexetil, MCP-I, sP-选择素, CD40, CD40L
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