| Trogopterus dung and Panax ginseng, is one pair of nineteen herbs of mutual antagonism. Was prescription in common use in clinical practice. Experiments on compatibility of the tow medicines were practiced and summarized in this thesis.Chemical and pharmacology studies are tow main parts of the thesis.In chemical works, tow compounds were isolated from EtOAc fraction of trogopterus dung abstract they were Beta sitosterol and proto-catechuic acid. Three compounds were isolated from Ginseng abstract, they were saponin Rd, Rc and Rb1. Fat-soluble composition in trogopterus dung was analysed by GC-MS method,42 compounds were identified and 15 of them were fat-acid, with a content of 49.162 percent. Volatile oil in trogopterus dung was extracted by steam distillation. Four commonly used compatibility proportion of the tow medicines were compared,5:1,3:1,1:1 and 1:3. Variation of content of total flavonoid and saponin were determined by UV, while content of protocatechuric acid and other nine saponins were determine by HPLC. Results showed under polypharmacy condition, saponin in ginseng declined except Rb3, and there was no linear relationship between proportion and the content. Content of protocatechuric acid varied as change of compatibility proportion, under condition of 5:1,3:1 and 1:1, protocatechuric acid in extract had a higher content than in single trogopterus dung.In pharmacology study, antioxidation activity was tested by DPPH method. Activity of extracts from trogopterus dung by different solvent, volatile oil, and different compatibility proportion were compared, EtOAc and n-BuOH fraction of trogopterus dung had stronger activity in eliminating DPPH radical, and the activity decreased as gingseng goes to a higher proportion in compatibility. Bacteriostatic study was carried on colibacillus and staphylococcus aureus, all above groups performed inhibition, chloroform and ethyl acetate extract of trogopterus dung showed outstanding inhibition ratio, under 25 mg/ml condition, the ratio came to 60.06% and 57.81%. Volatile oil of trogopterus dung also showed inhibition activity, but was not significant. Both gingseng and trogopterus dung ethanol extract were able to inhibit colibacillus and staphylococcus aureus, while trogopterus dung group performed stronger. Proportion 5:1 came to the highest inhibition ratio at concentration of 25 mg/ml, the ratio was 73.55% and 60.58%, which was significant difference to the blank, that was even higher than single trogopterus dung. Our study manifested the following results:Compatibility of the tow medicines, affected content of some saponin in ginseng, and enhanced activity components in trogopterus dung. As proportion of panax ginseng went higher, activity of antioxidation and bacteriostasis declined, but some of them performed stronger. This indicates that, compatibility of trogopterus dung and panax ginseng is feasible in clinical use for treatment of trogopterus dung-treating diseases. |
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