Studies Of Chemical Constituents And Antioxidant Activity Of Jujubae Fructus In Xinjiang Province

Objective:To study chemical composition and antioxidant activity of six cultivars of jujubae fructus from different areas in Xinjiang province, provide a more stable and reliable method for quality control, and provide a reference for clinical medicine and food processing. Methods:Total flavonoids and Polyphenolic contents were measured using spectrophotometry; rutin, oleanolic acid, ursolic acid contents were measured using high performance liquid chromatography (HPLC method), cyclic adenosine monophosphate (cAMP) contents were measured using Rapid Resolution Liquid Chromatography (RRLC); to determine metal elements by Microwave Digestion-Flame Atomic Absorption Spectrometry(FAAS); to optimize the extract technology of total flavonoids from jujubae fructus; to study Separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin; utilise three types of antioxidant activity models (clear DPPH free radical, hydroxide radical and SOD active effect) do antioxidant activity screening to water extracts of jujubae fructus. Results:There were differents levels of total flavonoids and polyphenolic contents in six cultivars of jujubae fructus; the contents of rutin, oleanolic acid, ursolic acid and cyclic adenosine monophosphate in six cultivars of jujubae fructus in XinJiang existed obviouse difference; determined metal elements contents in six cultivars of jujubae fructus; through 2 times of refluxing with 70% alcohol, of which the amount was 12 times of sample, and 1h for each refluxing, the best extraction efficiency was obtained. experimental results of the static and dynamic adsorption-desorption experiments show that D101 resin possesses higher absorption and desorption capacity. The results of the present work indicate that the water extracts from six cultivars of jujubae fructus had different levels of antioxidant activity. Conclusion: The study determined contents of total polyphenolic, total flavonoids, rutin, oleanolic acid, ursolic acid, cyclic adenosine monophosphate and metal elements in six cultivars of jujubae fructus, optimized the extract technology of total flavonoids and studied separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin, to study antioxidative actions of water extracts from six cultivars of jujubae fructus in vitro, offered theory evidences for quality control and further study of jujubae fructus.