| Objective: To evaluate the effect of transcutaneous acupoint electrical stimulation (TEAS)on reproductive outcome in patients treated with in-vitro-fertilization (IVF)/intracytoplasmic sperm injection (ICSI),and through the analysis of the clinic pregnancy outcome for finding Optimal indications to mechanism research.Method: Clinical research:A total of 600 patients, less than 45 years old, undergoing cryopreservation embryos transplant or fresh cycle with IVF or ICSI .During the study period all patients receiving IVF/ICSI treatment were randomly allocated to five groups. The first group of patients received no treatment on the day of ET;The second group received once 30 min placebo treatment(mock HANS)on placebo acupoint after ET ;The third group received once 30 min placebo treatment(mock HANS)on acupoint after ET ;The fourth group received once HANS (2HZ,8~10 mA)treatment on acupoint after ET ;The last group received twice HANS(2HZ,8~10mA) treatment on acupoint on the ET day and yesterday before ET received twice HANS(2HZ,15~20HZ) treatmen.Mechanism research:Based on clinical pregnancy rates choose eligible patients in 40 cases, randomly allocated into two groups, each group 20 patients. In the intransplantation and wihtout medicine recuperates cycle for mechanism research, all patients were B ultrasonic monitoring ovulation, ovulation days monitoring endometrial thickness and type, The black group of patients received no treatment after ovulation, HANS twice treatment group received acupoint treatment during two to three days after ovulation,both groups take endometrial during 6 to 8 days after ovulation , compare endometrial Pinopodes and integrin alpha 1 beta 1, alpha 4 beta 1, alphaνbeta 3 level, explor the preliminary mechanism.Results:Clinical research :There was no difference between no treatment group and placebo group in pregnancy rates(p>0.05). Clinical pregnancy(47% vs 29%,p<0.05) and take baby home(41% vs 21%,p<0.05)and implation rates(25.7% vs 15.0%,p<0.01)were significantly higher in the once HANS treatment group as compared with the mock HANS real acupoint treatment group . The Biochemical (54% vs 34%,p<0.01)and clinical pregnancy rates (50% vs 29%,p<0.01),implation rate(25.9% vs 15.0%,p<0.01)and take baby home rate(42% vs 21%,p<0.01)in the twice HANS treatment group were significantly higher than the mock HANS real acupoint treatment group.The Biochemicaland clinical pregnancy rates,implation rate and take baby home rate in the twice HANS treatment group were significantly higher than the once HANS treatment group, but the difference did not reach statistical difference.Mechanism research:There was no difference between the blank group and the twice HANS treatment group in Pinopodes growing in rich(p=0.429); Pinopodes growing moderate(p=0.022<0.05) and poorer number (p=0.004<0.01)were significantly difference in blank group and the twice HANS treatment group.Integrin alpha 1 beta 1, alpha 4 beta 1 and alphaνbeta 3 levels were higher in the twice HANS treatment group than blank group ,and the difference did not reach statistical difference,but has an upward tendency.Conclusion:Transcutaneous acupoint electrical stimulation on the day of ET significantly improves the reproductive outcome of IVF/ICSI,compared with no treatment and placebo treatment.Transcutaneous acupoint electrical stimulation can induce Pinopodes open and maybe improve integrin alpha 1 beta 1, alpha 4 beta 1 levels in endometrial planting window period ,so it may affect the setup of endometrial receptivity. |
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