Relationship Between Maternal PAHs Exposure, XRCC1 Polymorphism And Newborn Growth And Development

Objective To know more about the PAHs exposure level of pregnant and understand the growing development situation of new-born in Taiyuan city. To explored the impact factors of pregnant exposure level to Paths and the newborn growth and development. To further explore the relationship between DNA repair gene polymorphism and the newborn growth and development, and to investigate the interactions of PAHs exposure level and DNA repair gene polymorphisms. In a word, this study was designed to provide a theoretical basis for developing effective polices related to environmental health, as well as to provide a scientific references for selecting susceptible population, controlling environmental pollution and conducting researches related to prevent the prenatal exposure to PAHs.Methods1. To indentify Taiyuan city as the research field according to the past air monitoring data and related documents at home and aborad.2. The basic information, including smoking and drinking, residential and environmental exposures, disease and pregnant history, was collected by a detailed and validated questionnaire. The data about newborn development indexes and was amassed from hospital.3. Sampling and measuring3.1 The content of 1-OHPy in pregnant' urine was measured using enzyme hydrolysis-High Performance Liquid Chromatography (HPLC).3.2 Using the RFLP-PCR to detect XRCC1 Arg194Trp, XRCC1 Gln632Gln gene polymorphisms.Results1. In this study, the percentage of detectable samples in urine was 84.9%,85.8%of the maternal urine 1-OHPy level were higher than that in the common residents(0.11μmol/molCr) and 13.0% of the maternal urine 1-OHPy level were higher than the exposure limit of Coke-oven workers(1.9μmol/molCr).2. The category of energy, cooking frequencies and if have a smoke exhaust system in kitchen are the independent factors to the 1-OHPy level (p<0.05).3. The multiple linear regressions model results showed that the residence heating mode and cooking frequencies are the independent factors to the 1-OHPy level after controlling the other confounders in multiple linear regression models.4. Significant associations of PAHs exposure level and newborn development indexes were not observed in this study (P>0.05).5. There is no observed relation between single gene polymorphisms and newborn growing and development indexes (P>0.05).6. The interactions of PAHs exposure level and Arg194Trp gene polymorphism had significant difference in birth length(P=0.024). The birth length of newborn whose mother exposured to high PAHs level with mutant gene was significant lower than reference group(P=0.035). The interactions of PAHs exposure level and Gln6632Gln gene polymorphism had significant difference in birth length of newborn (P=0.040). And there are no observed interactions of PAHs exposure level and gene polymorphism in other newborn growth and development indexes (P>0.05).Conclusions1. In this study, the percentage of detectable samples in urine was 84.9%, of the maternal urine 1-OHPy level were higher than that in the common residents(0.11μmol/mol Cr) and 13.0% of the maternal urine 1-OHPy level were higher than the exposure limit of Coke-oven workers(1.9μmol/mol Cr). The results suggested that the subjects we had chosen had been exposed to high level PAHs.2. Cooking fumes may be the main factors of pregnant women exposure to PAHs. The family energy category, cooking frequencies and kitchen exhaust system are the main way of their exposure to PAHs. Therefore, effectively measures, such as using kitchen exhaust system and kitchen separated from the sitting room or living room and so on, should be taken to improve the residence air quality, these measures are especially important to pregnant women.3. Significant associations of the exposure ways to PAHs (such as Life environment, habit and so on) and newborn development indexes were not observed in this study.4. Newborns birth length is more sensitive than birth weight to gene-environment interaction, and it can be considered as a more ideal indicator of evaluation the impact of prenatal exposure to environmental pollutants on offspring growth and development.5. The interactions of PAHs exposure level and Arg194Trp gene polymorphisms had significant difference in birth length, and the interactions of PAHs exposure level and Gln632Gln gene polymorphisms also had significant difference in birth length of newborn.