| ObjectivesThere is a continuous process between endometrial hyperplasia and endometrioid adenocarcinoma. Endometrial hyperplasia includes simple hyperplasia, complex hyperplasia, and atypical hyperplasia, which can develop into endometrioid adenocarcinoma with different percentages. This experiment was to analyze the expression of NF-κB p65, ERαandβ-catenin in different tissues of endometrium, aiming to investigate the possible pathogenesis in endometrioid adenocarcinoma furtherly.Methods90 cases of different tissues of endometrium underwent curettage at Tianjin Medical University General Hospital between 2006 and 2008 were retrospectively retrieved, including 10 cases of normal endometrium,60 cases of endometrial hyperplasia and 20 cases of endometrioid adenocarcinoma. Two-step immunohistochemical staining was used to detect NF-κB p65, ERαandβ-catenin in different tissues of endometrium. All statistical analyses were preformed using the statistical package SPSS 13.0, including One-Way ANOVA and Kruskal-Wallis test, two independent samples q test and Mann-Whitney test, and Spearman correlation analysis. P<0.05 was considered to have statistic disparity.Results1. In 3 groups of different endometrium tissues, the positive rates of NF-kB p65 was respectively 0.00(2.71)%,0.47(15.25)% and 9.13 (48.90)% in the form of [Median (Quartile)]. There was stasistical difference between them (Z=26.792, P<0.01). In 3 groups of different endometrial hyperplasia, the positive rates of NF-κB p65 gradually raised from simple hyperplasia, complex hyperplasia to atypical hyperplasia, with stasistical difference among those 3 groups (Z=18.826, P<0.05). In 20 cases of endometrioid adenocarcinoma, there was no stasistical difference in positive rates of NF-κB p65 between different ages, pathology grade and whether differentiation into squamous epithelium or not (P>0.05).2. In 3 groups of different endometrium tissues, the positive rates of ERαwas respectively 5.25±3.96%,49.97±22.13% and 35.01±20.91% in the form of (x±s). There was stasistical difference between them (F=21.299, P<0.01). In 3 groups of different endometrial hyperplasia, the positive rates of ERa gradually reduced from simple, complex to atypical hyperplasia, with stasistical difference among them (P<0.05 or P<0.01). In 20 cases of endometrioid adenocarcinoma, ERa positive rate was significantly higher in cases with lower pathology grade (P<0.05). There was no stasistical difference in positive rates of ERa between different ages and whether differentiation into squamous epithelium or not (P>0.05).3. In 3 groups of different endometrium tissues, the positive rates of P-catenin was respectively 0.00(0.36)%,1.75(51.40)% and 20.03(68.45)% in the form of [Median (Quartile)]. There was stasistical difference between them (Z=14.360, P<0.01). In 3 groups of different endometrial hyperplasia, the positive rates of P-catenin gradually raised from simple hyperplasia, complex hyperplasia to atypical hyperplasia, with stasistical difference among those 3 groups (P<0.05 or P<0.01). In 20 cases of endometrioid adenocarcinoma,β-catenin positive rate was significantly higher in cases with differentiation into squamous epithelium (P<0.05). There was no stasistical difference in positive rates of P-catenin between different ages and pathology grade (P>0.05).4.In 20 cases of endometrioid adenocarcinoma, there was a negative correlation between ERa,β-catenin and NF-κB p65(r=-0.491, P=0.028 and r=-0.517, P=0.020), but no correlation was noted between ERa andβ-catenin.Conclusions1. The expression of NF-κB p65 was significantly higher in endometrioid adenocarcinoma. Through anti-apoptosis and promoting proliferation, NF-κB p65 may play an important role in endometrioid carcinogenesis and its development.2. The expression of ERa was significantly lower in endometrioid adenocarcinoma. As the pathology grade increasing, the expression of ERa was decreased.The results implied that ERa was a useful indicator for diagnosis and prognosis.3. The expression ofβ-catenin was significantly higher in endometrioid adenocarcinoma, which indicated thatβ-catenin may have a role in the early stage of endometrioid carcinogenesis. Detection ectopic expression ofβ-catenin may be an accuracy and effective method in diagnosis using immunohistochemistry. In addition, the expression of P-catenin was increased in cases with differentiation into squamous epithelium, which certified the overexpression of P-catenin may furtherly facilitate endometrioid adenocarcinoma differentiating into squamous cell carcinoma.4. NF-κB p65 may have an inhibitory cross-talk with ERa in endometrioid adenocarcinoma, which made carcinoma developing into the status independent of hormone.5. There was also a molecular cross-talk between NF-κB p65 and P-catenin in endometrioid adenocarcinoma, which resulted to a negative correlation between them, and ended in the transition of specialty tumor cell phenotype. |
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