Toxicological Evaluation And Lipid - Lowering Function Of Tobacco Seed Oil

Objective:To evaluate the toxicity of tobacco seed oil by acute oral toxicity test, three tests of hereditary toxicity and thirty days feeding test. To study the lipid-lowering effect of tobacco seed oil by the model of SD rats fatty.Methods:1. The horn method was adopted for acute oral toxicity test in mice, calculating the median lethal dose(LD50)of tobacco seed oil to the mice. 2. To observe the number of revertant colonies of salmonella typhimurium in different group of tobacco seed oil by Ames test. 3. To compared the micronucleus rate in different group of tobacco seed oil by the bone marrow eosinophils dyed red cell micronucleus test. 4. To compared the chromosome aberration rate in different group of tobacco seed oil by the bone marrow eosinophils dyed red cell the chromosome aberration rate test. 5. Choose 80 SD rats,according to the weight of rats randomly divided into 4 groups, were blank control group,low, medium and high dose of tobacco seed oil group(5、10、20g/(kg﹒bw﹒d)), each group had 10. To give the tobacco seed oil into their feed, according to 10% of body weight reduced, the subjects content dose gived per 100 g weight dose of quantity discount for feed. After 30 days, 12 h fasting, rats was anesthetized by pentobarbital sodium after they were weighted and blood was collected. Then weighted their liver,kidney, spleen, testis, ovary weighing, calculating the orgab body ratio and observed for histopathology by HE staining. 6. Model of fatty rats building and animal grouping:choose 60 SD rats, according to the weight of rats randomly divided into 5 groups, each group had 12 rats. A group was the basis of the control group, gavaged 2 ml/(kg?bw?d) of distilled water every day and ordinary feed; For high fat control group, group B was gavaged daily 2 ml/(kg?bw?d) of distilled water, high-fat feed. C, D, E for tobacco seed oil of low, medium and high dose group, gavaged tobacco seed oil 2, 4, 6 ml/(kg﹒bw﹒d)every day, high-fat feed. After 6 weeks, 12 h fasting, rats was anesthetized by pentobarbital sodium.Calculated the Lee’s index after they were weighted and measured.blood was collected to detetion the biochemical indicators and calculated the AI index.Then stripped the fat around the kidney、genitals 、mesenteric tocalculate the fat body ratio.The liver wsa obtained for histopathology by HE staining.Results: 1.Poisoning and death did not occur in mice within 14 days, the median lethal dose(LD50)> 21,500mg/kg. 2. Ames test showed that the number of revertant colonies for each dose group of the tested sample was two times less than spontaneous number of revertant colonies, also had no dose-response relationship occurred. With or without S-9,genetic toxicity of salmonella typhimurium TA97, TA98, TA100 and TA102 strains had not been present 3. Compared with the positive control group, the micronucleus rate of each tobacco seed oil treated group was significantly decreased, there are significant difference(P < 0.05). The micronucleus rate of the positive control group is significantly higher than the negative control group, there are significant difference(P < 0.05). 4.Compared with positive control group, the chromosome aberration rate of high tobacco seed oil group was significantly decreased(P < 0.05), and that in low dose group and medium dose group were not changed significantly. The chromosome aberration rate of the positive control group is significantly higher than the negative control group, there are significant difference(P < 0.05). 5. After feeding for 30 days, there was no poisoning and death happened in experimental mice. Body weight, food utilization and organ-body ratios had no significant difference with the control group; the blood and blood biochemical indexes ALT, AST, BUN, Cr, GLU, ALB, TP, TC, TG were within the normal range and had no statistically difference compared with the respective control group; In addition, histopathologic examination showed that liver tissue in the high-dose group could been observed a few of fat vacuoles, but the other main organs of mice in other groups had no pathological changes 6. Compared wih high fatty group, low,medium, high dose group of tobacco seed oil decreased obviously in fat body ratio; but the Lee’s index had no significant difference between each grop; AI value in high fat group was highest, the rest of the group AI were significantly lower, and with the increase of tobacco seed oil dose, AI value decreased more.Compared wih high fatty group, low,medium, high dose group of tobacco seed oil decreased obviously in TC, LDL- C content,there are significant difference; Compared wih high fatty group, low, medium,high dose group of tobacco seed oil not decreased obviously in TG content,there are no significant difference; Tobacco seed oil high dose group compared with blank control group obviously higher in HDL-C content, there are significant difference, the rest of the group no statistical difference; Compared wih high fatty group, low, medium, high dose group of tobacco seed oil decreased obviously in blood glucose index,there are significant difference. H.E staining observation results showed that tobacco seed oil after the intervention of tobacco seed oil, fat cells, fat cavitation, inflammatory infiltration and karyolysis high lipid control group significantly reduce compared with high fatty group.Conclusions: 1. According to the results of tobacco seed oil in acute oral toxicity test,three genetic experiment and 30 d feeding experiment, confirmed that the tobacco seed oil had no obvious toxic action within the scope of this study. 2. By the blood biochemical indicator detection and pathology observation in the rat model of high fat, confirmed that the tobacco seed oil had a certain activity of lipid.3.Through the toxicology evaluation of tobacco seed oil and lipid-lowering experiments, the results show that tobacco seed oil was expected to become a health care function of oil products, but the effect of human body remained to be further experiments and research.