Preparation And Application Of A New Class Of Brush Chiral Stationary Phase

Since the chiral drug enantiomers of biological activities and pharmacological properties are different, the chiral separation becomes a research hot spot in the field of separation and analysis. The method of High performance liquid chromatography(HPLC) has the advantages of convenience, high efficiency, rapidness and accuracy, and is one of the most commonly used method of the chiral drug separation and analysis.The stationary phase is the core of high performance liquid separation and analysis technology.Taking silica gel as the carrier, KH-566 and KH-550 as the linker, commonly used drug simvastatin and valsartan as chiral selectors, the two new brush chiral stationary phase was prepared in using bonding, simvastatin as chiral selectors of chiral stationary phase and valsartan as chiral selectors of chiral stationary phase, and by making use of TGA,FT-IR and elemental analyzers on the characterization and analysis, the obtained data showed that the synthesis of two new brush stationary phase achieved its original goals.Four enantiomers are successfully separated by the simvastatin as chiral selectors of chiral stationary phase(CSP-A).They are benzoin,nitrendipine,felodipine and 9-phenylxanthen-9-ol. We compared the separation results of benzoin enantiomer by the simvastatin as chiral selectors of chiral stationary phase(CSP-A) and relevant stationary phase, the results show that the separation results of this chiral stationary phase is superior to the relevant stationary phase. We also studied the stationary phase was prepared by forward reaction and reverse reaction to relevant enantiomers’ separation effect, and found the reverse bonded stationary phase with high carbon content, but the separation effect was not improved.Three enantiomers are successfully separated by the valsartan as chiral selectors of chiral stationary phase(CSP-B). They are celiprolol, atenolol and propranolol. And components of the mobile phase, composition of the mobile phase and flow rate of the mobile phase were investigated. We got the results that n-hexane:tetrahydrofuran is a better mobile phase for separating the three kinds of drugs and the better proportion of n-hexane:tetrahydrofuran is 80:20, and the better flow rate is 1.0 ml/min.