Ultrasonic Extraction Of Eucommia Powder Preparation Process And Eucommia Leaf Chlorogenic Acid Separation Study

A new method of producing extract powder of eucommia ulmiodes (EU) from EU leaves has been developed in this paper by using ultrasonic process at low temperature. The different raw materials, extractive process, and purification methods have been investigated systemically. The results showed that the extract powder produced by the technology of ultrasonic wave process at low temperature and in the following procedure of separation by ultra-filtration were much better than that produced by the traditional high temperature decocting extractive method. The content of effective ingredient, such as, trace elements, aminoacids and chlorogenic acid (CA) was enhanced significantly. The extract yield increased 56.7%, and the content of total sugar decreased by 35.3%. The quality of the powder products obtained by the new process has high stability, fine appearance, water-solubility and no agglomeration. The optimum conditions of the process were established in the frequency of ultrasonic wave 13kHz at the temperature 50 for 45 min, and the ratio of raw material to liquid 1 .' 8. When the later deposition process was performed with nature precipitant in stead of ethanol to the extract aqueous, we found that it not only decreased the cost in the following concentrate process but also avoided the second pollution caused by the organic solvent.Based on the process of extract powder of EU, another way of separation and purification of the main medical bioactive component of CA in the EU leaves was also investigated in this paper. The raw material selection, extractive process and purification method have been studied carefully. An effective method to analysis CA was established to satisfy the demand. It was found that the best raw material to extract CA is theEU leaves before falling down from the tree and dried in the shade. The solubility of CA in cold water is lower than in hot water, and it is easily dissolved in ethanol and acetone. Considering the facts of cost, quality of the CA, and solubility, warm water was chosen as extract solvent. Because of the hot sensitive property of CA, ultrasonic wave extracting at low temperature was used in the work. The extract yield was increased and the availability component avoided to being destroyed as well. The macro-molecule such as protein were removed by using the Ultra-filtration. The advantage of this process is significant, for example, there is no phase transformation and no need of any organic solvents. The CA can not be decomposed under this conditions, the loss of extract in this step is low too. The Macro-reticular Resin was used to purify CA in the last procedure. The conclusion was obtained that it was simple to operate, and the material in the column was easy to regenerated, at the same time, it could increase the purity of CA when using the new method.