Study On Effect And Mechanism Of Analgesia On Adjuvant-induced Arthritis Rsts With Crossing Selection Of Acupoints Method

Objective: Crossing selection of acupionts method is one of the acupoint-selecton methods recorded in HuangDi internal classic and is still applied in acupuncture clinic today, which clinical effect and mechanism are still need to be elucidated. This study is to discuss the effect and part of center mechanism of analgesia on adjuvant-induced arthritis rats of this method.Methods: A total of 50 SD rats were randomized into 5 groups , they are normal group, model comparison group, right-hind points group, left-hind points group, left-fore points group, with 10 animals in each group. Acute inflammation pain model was established by injecting Complete Freund's adjunvant, taking electroacupuncture as stimulating technique, selecting"Zusanli"and"Yongquan"at right-hind limb as pricking position for model comparison group, selecting"Zusanli"and"Yongquan"at left-hind limb for left-right crossing prick group, selecting"Quchi"and"Hegu"at right-fore limb for upper-lower crossing prick group, heating radiation was used to measure local pain threshold, radioimmunoassay to measureβ-EP , ACTH in hypothalamus and Dynal in medulla so as to observe the analgesia effect of different crossing acupoint selecting methods from usually used one.Results: Better analgesic effect has been observed in Both normal acuponits selection group and crossing acupoints selection group. Compare two kinds of crossing acupoints selection method, the left-right one is better. Content ofβ-EP, ACTH and Dynal decrease in normal acupoint selection method group and left-right crossing acupoints selection method group in rats. Content ofβ- EP, ACTH decrease in upper-lower crossing acupoints selection group but there's no obvious difference about Dynal content between this group and model group.Conclusion: Better analgesic effect of all of normal acupoints selection method, left-right crossing selection of acupoints method and upper-lower crossing selection of acupoints method has been abserved in the study, and the left-right crossing selection of acupoints method is the best. Parts of the effective mechanism is probably stimulating the regulation function of analgesic material in central nervous system.