Methylation Alterations Of Mir-34 Gene And Its Significance Of Hazakh's Esophageal Squamous Cell Carcinoma In Xinjiang Province

Objective:Detect the methylation conditions of miR-34 gene in Hazakh's esophageal carcinoma in XinJiang Province, to explorer the relationship and significance between Hazakh's esophageal carcinoma and the methylation of miR-34 gene.Methods:Using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis to detect the methylation conditions of 145 cases of Hazakh's esophageal squamous cell carcinomas(ESCC),60 cases of non-cancer tissues adjacent to esophageal squamous cell carcinomas(NCAE) and 39 cases of mucous membrane of normal esophagus(NE); Using software of Cluster 3.0,TreeView and GraphPad Prism 5.0 to analysis the distribution of the CpG units among the ESCC,adjacent and NE; Using SPSS 17.0 software package to do all the statistical analysis.Results:(1) our results showed that the methylation of miR-34 gene in ESCC is higher than the NCAE and NE. (2) Comparison of the methylation rate among ESCC,NCAE and NE,there were 5 units(CpG₁.2.3, CpG₉.10, CpG₁1.12.13, CPG₁4, CpG₁5.16) among the 8 CpG units that could be detected had statistical significance(p<0.05)(3)Comparison of the methylation of different rate among well-differentiated,moderately or poorly differentiated ESCC,the methylation difference (moderately-poorly differentiated> well-differentiated) of miR-34b/c CpG₁.2.3 had statistical significance(p<0.05); Comparison of the methylation rate between different TNM staging, the methylation difference (â… +â…¡>â…¢+â…£)of miR-34b/c CpG₉.10,CpG₁1.12.13 had statistical significance(p <0.05);Comparison of the methylation rate between gender,age,position,gross, position,gross,lymph node metastasis and HPV16 infection, there were no CpG unit had statistical significance(p>0.05).Conclusions:(1) the methylation of miR-34 gene in Hazakh's Esophageal squamous cell carcinomas is higher than non-cancer tissues adjacent to tumor and normal control,indicate that mthylation of miR-34 b/c gene may be involved in the pathogenesis of Hazakh's esophageal carcinoma in XinJiang Province.(2) Hypermethylation of miR-34b/c CpG ites CpG₁.2.3 maybe correlated with Hazakh's esophageal carcinoma pathology differentiation,higher methylation in poor differentiation. and hypermethylation of CPG₉.10,CpG₁1.12.13 maybe correlated with Hazakh's esophageal carcinoma clinical staging,indicate that involved in the tumor earlay progress.