Danshen Injection The Treatment Of Chronic Pancreatitis

Objective:Chronic pancreatitis (CP) is due to pancreatic repeated or persistent inflammation of the pancreas and pancreatic duct led to progressive and irreversible damage and fibrosis, the main symptoms are abdominal pain, weight loss, diabetes, jaundice and steatorrhea, and performance different levels inside and outside the secretion of pancreatic dysfunction or loss. The morphological features of chronic pancreatitis is the progressive pancreatic fibrosis, a typical pathological changes of pancreatic tissue is reduced, irregular nodular hard, often accompanied by pancreatic tissue calcification, or acinar atrophy, pancreatic ductal Festival segment expansion, it can have within the formation of stones and pseudocyst, pancreatic islet cells associated with reduction or atrophy. The pathophysiologic process more complicated, mainly due to pancreatic tissue in the collagen-based extracellular matrix (ECM) synthesis and precipitation increased, decreased degradation, both due to loss of homeostasis. In this study, by measuring laminin (LN) and hyaluronic acid (HA), which verifies the SM treatment of chronic pancreatitis and different doses of Danshen injection on the treatment of chronic pancreatitis. In this study, using photometric enzyme-linked immunosorbent assay (ELISA) detection of normal SD rat pancreas, chronic pancreatitis control group, Danshen injection intervention low dose group, Danshen injection interventionMethods:SD rats were randomly divided into 5 groups, etc., n=10, respectively, the normal group, model control group, low dose SM intervention group (400mg/kg), the dose of Salvia intervention group (800mg/kg), large doses of Salvia intervention group (1600mg/kg). In addition to the normal group, the other 4 groups of preoperative fasting is not water 12h, amiodarone (100 mg/kg body weight) by intraperitoneal injection of anesthesia, rats were fixed routine disinfection, to do the white line along the ventral midline incision, 41 2Scalp vein needle on the 2nd after the bile duct, pancreatic duct near hilar clamping end, through the constant flow pump to the injection of 2% of duodenal papilla trinitrobenzene sulfonic acid (TNBS) ethanol phosphate buffer 0 4ml, 1h Note finished, close the current pump to maintain the pressure after 10min, to prevent the drugs back, layer by layer close the abdominal cavity, strict aseptic surgical procedure. To prevent dehydration in rats, daily subcutaneous injection of saline 2 times, each time 6ml, until the rats were able to free access to water. Given routine postoperative diet after 72h fasting.72h after the operation with different doses of Danshen injection to 4 weeks after intramuscular injection. The rats were sacrificed, blood specimens from serum,-20℃preservation; take pancreatic tissues were fixed with formalin. Room temperature before the experiment will be re-dissolved in serum set, turn centrifugation, the supernatant were measured. By photometric enzyme-linked immunosorbent assay (ELISA) detection of the normal pancreas group and the control group and intervention group in the LN and HA levels, and by HE staining of fibrosis in chronic pancreatitis changes.Results:Chronic pancreatitis in the control group significant pathological changes of pancreatic tissue. Intraductal injection of 2% TNBS immediately found congestion and swelling of pancreatic tissue, rats were killed after 4 weeks, remove the pancreas, the pancreas can be seen significantly smaller size, texture, hardening, and the blurred boundaries with the surrounding tissue, high-power microscope, interstitial inflammatory cell infiltration of pancreas, a large number of fibrous tissue hyperplasia, dense collagen and fibroblasts to islet cell division; pancreatic acinar foam cell loss and some vacuolar degeneration, shrunken cell bodies calcification, pancreatic duct hyperplasia expansion of perivascular fibrosis, perivascular and interlobular and intralobular pancreatic fibrosis with inflammatory cell infiltration. Salvia also occurred in the intervention group, pancreatic fibrosis, but the SM group than in model group intervention significantly reduced fibrosis, and with increasing doses of Danshen injection of pancreatic fibrosis were gradually reduce the trend. The five rat pancreatic tissue biopsy for fibrosis score, that score higher than the control group, the intervention group Salvia, a higher degree of fibrosis. ELISA, the test showed that:LN and HA in the intervention group were significantly lower than the control group, and the difference was significant.Conclusions:Danshen injection in rats with chronic pancreatitis and pancreatic tissue against fibrosis. By Danshen injection on the treatment of chronic pancreatitis and fibrosis, can be obvious from the pathological changes observed in pancreatic fibrosis of chronic pancreatitis decreased, while chronic pancreatitis serum LN and HA were significantly reduced.