"compound Of Chinese Herb Extracts Of Movement In Rat Brain Tissue Metabolism Of Free Radical Research

1 Objective:The key point of this paper is to investigate the effect of "Compound extract of Chinese medicine" on brain free radical metabolism of the exercise training rat, to understand free radicals metabolism characteristics in brain and the mechanism of free radical scavengers under different functional states. And to provide the scientific basis of "Compound extract of Chinese medicine" for better service in the movement practice and guiding nationwide fitness.2 Method:140wistar rats were randomly divided into four groups:N(normal control group), T(training control group), M("compound antioxidants" supplementation), TM(training+medicine group). each group was feed with normal animal food. After a week of feeding, T group and TM group were both trained for 8 weeks. The rat of taking medicine was given" Compound extract of Chinese medicine" agents eight weeks. After 9 weeks, the four groups were randomly divided into five sub-groups, group of seven, and kill them in five condition (the rest state, the immediate ends of the fixed load, the immediate ends of exhaustive exercise and 12,24 hour ends of exhaustive exercise). And the activity of MDA, GSH-PX, GSH, SOD, T-AOC of the rat brain were measured. All of the data have been processed by SPSS 17.0 software package with T-check for the comparison between groups and p<0.05 is taken as the standard.3 Result:3.1 In rest state, MDA content in brain, T, M and TM group was significantly lower than N group (P<0.01); GSH—PX activity, T group was significantly higher than N group(P <0.05), TM group was significantly higher than the N group (P<0.01), between other groups were no significant difference (P>0.05); GSH content and SOD activity, T and TM group was significantly higher than the N group (P<0.01), and TM group was significantly higher than the T and M group(P<0.05); T—AOC activity, M and TM group was significantly higher than N group (P<0.01); TM group was significantly higher than the T and M group(P<0.05).3.2 At the immediate ends of the fixed load, MDA content in brain, T,M and TM group was significantly lower than the N group (P<0.01), TM was significantly lower than M group (P O.05); GSH-PX activity, T,M and TM group was higher than the N group (P<0.05), T group was very significantly higher than M group (P<0.01); GSH content and T—AOC activity, M and TM group was very significantly higher than the N group (P<0.01), T group was higher than the N group (P> 0.05); SOD activity, T and TM group was higher than N group(P <0.05), M group was higher than N group (P> 0.05).3.3 At the immediate ends of exhaustive exercise, MDA content in brain, T,M and TM group was significantly lower than N group (P<0.01), TM group was significantly lower than M group (P<0.05); GSH—PX activity, TM group was significantly higher than N group (P<0.01), T and M groups was no significant difference than N group; GSH content, T,M and TM group was significantly higher than N group (P<0.01), T and TM group was significantly higher than M group (P<0.01); SOD activity, T, M and TM group was significantly higher than N group (P <0.01), between the three was no significant difference; T—AOC activity, TM group was significantly higher than M,T and N group (P<0.01),M group was significantly higher than N group (P<0.05).3.4 At the 12 hours ends of exhaustive exercise, MDA content in brain, T,M and TM group was significantly lower than N group (P<0.01); GSH—PX activity, T group was significantly higher than N group (P<0.01), GSH—PX and SOD activity, M and TM group was significantly higher than the T group (P<0.01), T and N group were no significant difference (P> 0.05); GSH content, T and TM group were all significantly higher than N and M group (P<0.05); T—AOC activity, M and T group was significantly higher than N group (P<0.01), TM group was significantly higher than T and M group (P<0.05).3.5 At the 24 hours ends of exhaustive exercise, MDA content in brain, T,M and TM group was significantly lower than the N group (P<0.05); Other four items, T,M and TM group were all significantly higher than the N group (P<0.01), and SOD,T—AOC activity and GSH content, TM group was all significantly higher than the N,M and TM group(P<0.01); GSH content, T and TM group was significantly higher than the M group(P<0.01).4 Conclusions:4.1 The "Compound extract of Chinese medicine" can reduce the MDA centent of the rat brain at different functional status, and reducing free radical damage to the brain4.2 The "Compound extract of Chinese medicine" could raise the GSH—PX,SOD,T-AOC activity and GSH content and enhance the anti-oxidation ability of the brain at different functional status.4.3. The "Compound extract of Chinese medicine" collaborative exercise training is more obvious than the simple compounding antioxidants supplementation group and simply training group for improving the activity of various antioxidant enzymes.