"yaotongding Injection Epidural Injection Of Rabbits Chemical Lumbar Radiculitis

ObjectiveTo determine the effects of epidural injection of YaoToagDiag Injection on mimic lumbar radicalitis in rabbits by using YaoTongDing Injection to inject around the lumbar nerve roots by the catheters, which were implanted in the epidural spaces, observing Model rabbits' affected extremity motor function and lumbar nerve roots' pathological changes and measuring 5-HT, histamine and PGE2 in tissue around the lumbar nerve root .MethodsSeventy-two common-grade adult white rabbits ,male and female in half, were mimicked lumbar radiculitis by implanting filter paper soaked the formalin around them, mimicking lumbar intervertebral disc herniation; and randomly divided into three groups: Model group (group A), YaoTongDing group(group B), Prednisolone group(group C). They were injected corresponding drugs once a week postoperation by the catheters, which were implanted in the epidural spaces. Model group(group A) were injected Saline injetion; YaoTongDing group(group B) YaoTongDing injetion; Prednisolone group(group C) Prednisolone injetion. 8 Model rabbits of all group were randomly put to death on the 7th, 14th and 21st day post-injection. Model rabbits' signs were observed on the 1st ,7th, 14th and 21st day post-injection. Model rabbits' affected extremity motor function and lumbar nerve roots' pathological changes were observed; 5-HT, histamine and PGE2 in tissue around the lumbar nerve root were measured on the 7th, 14th and 21st day post-injection. Experimental data were disposed through T-test, with the P<0. 05 as the standard of striking difference. The statistical results of detectingworth were expressed using the mean ?standard deviation (X ±S). Results1 Gait observation The affected extremity motor function of Group B and C were more obvious recovery than that of Group A on the 7th, 14th and 21st day, and the difference was significant (NP<0. 05 or nP<0. 01) . Those of Group B and C on the 21st day were more obvious recovery than those on the 7th day, and the difference was significant (bP<0. 01) .That of Group B between Group C on the 7th , 14th and 21st day was not so significant (CP>0. 05 ) .2 5-HT, histamine and PGE2 in tissue around the lumbarradiculitis5-HT, histamine and PGE2 of Group B and C were more obviously decreased than those of Group A on the 7th, 14th and 21st day, and the difference was significant ("P<0. 05 or "P<0. 01) . Those of Group B and C on the 21st day were more obviously decreased than those on the 7th day, and the difference was significant (bP<0. 01) . That of Group B and C on the 21st day was not so significant (CP>0. 05) .3 Pathological sectionInflammatory reactions of Group B and C were more obviously relieved than those of Group A.ConclusionsYaoTongDing injection has good effects on mimic lumbar radiculitis in rabbits. It can inhibit inflammation of the mimic lumbar radiculitis, and promote the injured lumbar nerve root to recover. Experiment expresses YaoTongDing injection has adjustment function on the inflammation of mimic lumbar radiculitis, and promote the injured lumbar nerve root to recover. The drug can decrease the inflammation mediums in the local constitution. The strength of it is approach that of Prednisolone injetion, which infers that the drug can replace the glucocorticoid drug for the epidural space injection.