| Objective: To investigate the homocysteine (Hcy) levels and the C677T polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR), a crucial factor of the Hey metabolism, and clarify the relationship between Hcy levels and high sensitivity C reactive protein (hs-CRP).Methods: By using the case-control study, 82 patients with type 2 diabetes mellitus were consecutively enrolled. 40 healthy controls were matched for age and sex. The MTHFR genotype was analyzed by PCR followed by Hinfl digestion. Homeostasis model assessment index (HOMA-IR) was calculated to estimate insulin resistance; Plasma total homocysteine levels were measured using the fluorescence polarization immunoassay technology. Plasma high-sensitivity CRP was measured using a particle-enhanced immunoturbidimetric assay.Results: Genotypic analysis revealed that the frequency of the mutation T allele in type 2 diabetic patients was 39.0%. The genotype distribution did not differ betweenthe control subjects and the type 2 diabetic patients ( x 2 = 0.668, P=0.709). Patientswith HOMA-IR exceeded the 50 percentile was defined as the high insulin resistance group. Logistic regression showed that the T allele was a risk factor to the insulin resistance (OR=5.906 P=0.03). Plasma tHcy concentrations and hs-CRP levels were significantly higher in patients with TT homozygous than in those with CC homozygous. Multivariate linear regression analyses revealed a strong independent association between tHcy and hs-CRP.Conclusion: There are no difference in the distribution of the C677T mutation of MTHFR gene between the Chinese type 2 diabetes patients and the healthy controls. The MTHFR gene T allele is one of the genetic predispositions to develop insulin resistance. In the patients with TT homozygous, Plasma tHcy levels and hs-CRP concentrations are greater than the CC homozygous patients. Plasma hs-CRP is an independent determinant of plasma tHcy. |
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