Association Of CD40-1C/T Gene Polymorphism With Serum SCD40L And Carotid Artery Plaque Instability

Objective:To investigate whether CD40-1C/T gene polymorphism is associated with serum soluble CD40L (sCD40L) expression and the instability of carotid artery plaque.Methods:According to the inclusion and exclusion criteria,select209cases of acute large artery atherosclerotic cerebral infarction as the case group, case group were further divided into the unstable plaque subgroup(61cases), the stable plaque subgroup (79cases), no plaque subgroup (69cases) according to the carotid ultrasound results.Select87cases of the same period without a history of stroke as a control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology was used to detect CD40-1C/T gene polymorphism and then sequenced, double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of serum sCD40L.Results:(1) CC genotype of the patient group was significantly higher than that in the control group (31.6%vs17.2%;χ2=6.353, P=0.012); C allele frequency was significantly higher than that in the control group (53.8%vs39.1%;χ2=10.689, P=0.001);serum sCD40L concentration was significantly higher than that of the control group [(3.97±1.20) vs (2.69±0.88); t=-10.194, P<0.001].(2) Unstable plaque subgroup CC genotype was significantly higher than that of stable plaque subgroup (55.7%vs31.6%;χ2=8.194, P=0.004) and no plaque subgroup (55.7%vs11.6%;χ2=28.849, P<0.001), stable plaque subgroup significantly higher than no plaque subgroup (31.6%vs.11.6%;χ2=8.547, P=0.003); the unstable plaque subgroup C allele frequency was significantly higher than stable plaque subgroup (77.1%vs54.4%;χ2=15.341, P<0.001) and no plaque subgroup (77.1%vs32.6%;χ2=51.401, P<0.001), stable plaque subgroup significantly higher than no plaque subgroup (54.4%vs32.6%;χ2=14.218, P<0.001); the unstable plaque subgroup serum sCD40L significantly higher than the stable plaque,and both of them were significantly higher than that of no plaque subgroup [(4.63±1.15) vs (4.19±0.99) vs (3.13±0.98); F=36.815, P<0.001)].(3) Multivariate logistic regression analysis showed that hypertension, type2diabetes, increased total cholesterol level, increased low density lipoprotein leve and C allele are the independent risk factors of large artery atherosclerotic cerebral infarction.Conclusion:(1) CD40-1C/T gene polymorphism is associated with the expression of serum sCD40L, C allele may increase the level of serum sCD40L expression.(2) CD40-1C/T gene polymorphism is associated with carotid artery plaque instability,C allele increased plaque vulnerability risks, eventually leading to cerebral infarction occur.