Expression Of SPAG9in Patients With Serous-epltnellal Ovarian Tumor

Background:Ovarian tumor is one of the common tumors in the female reproductive system, and its high incidence and mortality threaten women’s health and lives severely. Originated from the germinal epithelium of ovary, epithelial ovarian cancer accounts for50-70%of primary ovarian tumors, and its malignant type occupies the ovarian cancer85-90%. The serous ovarian tumor is the most common histological type of epithelial ovarian cancer. Due to the lack of early clinical performance, about70%of patients with ovarian tumor are diagnosed at advanced stages and the five-year survival is only30-40%. Consequently, exploring serum biomarkers and searching the function mechanism will be very important to the early diagnosis and prognosis evaluation.Objective:To investigate the tissue expression of sperm-associated antigen9(SPAG9) in benign ovarian epithelial tumors, borderline serous epithelial ovarian tumors and serous ovarian carcinomas. To detect the levels of serum SPAG9in patients with epithelial ovarian tumor. In order to evaluate the diagnostic value of SPAG9in serous tumor of ovary, and research the relationship between SPAG9and serous epithelial ovarian tumors.Methods:1. Subjects: 83cases tissues of ovarian tumors were collected from surgical patients, including the benign ovarian epithelial tumor(n=24); borderline serous epithelial ovarian tumor(n=27); serous ovarian carcinoma(n=32). Also collected some cases of pre-operative serum samples.2. Test:(1) Expression levels of SPAG9protein were retrospectively detected in paraffin sections from24benign ovarian epithelial tumors,27borderline serous epithelial ovarian tumors, and32serous ovarian carcinomas by immunohistochemistry. To clarify its expression levels and localization in tissues.(2) Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum SPAG9in patients with epithelial ovarian tumor.Results:1. The positive expression sites of SPAG9protein were mainly located in the cytoplasm and membrane of tumor, the intercellular substance has little or no expression. SPAG9expressions in the serous ovarian carcinomas was significantly higher than that of benign and borderline ovarian epithelial tumors(P<0.05).2. In ovarian carcinoma group, the average OD of SPAG9protein in highly and moderately differentiated carcinomas(44.0±10.88) is significantly higher than the poorly differentiated carcinomas(82.1±18.3)(P<0.05); it was higher in malignant ovarian tumor with lymphatic metastasis than that negativation, it has no significant difference(P>0.05).The expression of SPAG9protein was not related to age and clinical stage in the ovarian carcinoma group.3. The average serum level of SPAG9in the patients with ovarian carcinoma(80.52±35.96ng/ml) was significantly higher than that in the patients with benign tumors (2.48±1.20ng/ml)(P<0.05)4. Statistical analysis showed a positive correlation relationgship between the serum level of SPAG9and CA125(P<0.01).ROC curve revealed that SPAG9has a high significance to serous-epithelial ovarian tumor.Conclusion:1. SPAG9protein with high expression in serous-epithelial ovarian tumor, the expression levels of ovarian carcinoma group is obviously higher than benign and borderline ovarian epithelial tumors.2. In the ovarian carcinoma group, the expression of SPAG9protein in highly and moderately differentiated group is significantly higher than the poorly differentiated group. And it was associated with the grade of tissue differentiation3. Serum SPAG9levels in serous ovarian carcinomas are statistically higher than that in benign epithelial ovarian tumors. And it has a positive correlation relationship with the serum level of CA125.4. It may serve as a new type of epithelial ovarian tumor markers for early diagnosis and post-operation treatment.