The Mechanism Research Of Promoting Blood Irculation And Removing Blood Stasis Of San U Dripping Pills

Object:This topic through observation of San Ku dripping pills affects on different typesof blood stasis syndrome model rats,and achieving the influence blood agglutination andblood rheology of San Ku dripping pills,activating blood circulation and removing stasis onmyocardial injury of San Ku dripping pills and protective effect of related mechanism.Methods:Lusing arteral thrombosis instrument by local electrical stimulation ofinjured rat carotid artery methods of replication model of arterial thrombosis,effects ofdifferent does of tested drugs per unit of time （3min） on arterial thrombosis（arterial clogging degree%） effect.2with artery-vein bypass thrombosis method,throughthe bypass flow of thread forming thrombus weight, and plasma thrombintime （TT）, plasma prothrombin time（PT）, fibrinogen（FIB） comparison ofchange,oberved San Ku dripping pills on animal thrombosis formation in vivo effects,3withadrenaline and ice water stimulation factors for inducing blood stasis rats animal model ofblood stasis,observed in San Ku dripping pills on animal model of blood stasis of blood withdifferent shear rate viscosity changes and changes in plasma viscosity.4by ligation ofthe left anterior descending coronary artery induced myocardial ischemia and bloodstasis rats model, observation of San Ku dripping pills on myocardial blood damage（serum creatine kinase （CK）, amino acid transferase （AST）, lactatedehydrogenase （LDH）NO/ET-1） and thrombosis related factors （serum changes ofthromboxane,prostaglandin）.Results:1, on arterial thrombosis effects were demonstrated, San Ku dripping pillshigh dosage group,middle dose group of arterial thronbosis（arterial clogging degree%）was significantly lower than that of control group （p<0.05,0.01）, the tips of the San Kudripping pills on arterial thrombosis induced by electrical stimulation of the obviousinhibitory effect. In2, the artery-vein bypass thrombosis. Results show San Ku drippingpills, high, minddle, low does group of rat arterial thrombosis wet weight and dry weightin comparison with the control group were significantly different（P<0.05）. the tips ofSan Ku dripping pills on rats with artery-vein bypass thrombosis has an inhibitory effect.Each group of animal plasma TT, PT, FIB compared with the control group showed no significant difference, the tips of the San Ku dripping pills on TT, PT, FIB activity had noobvious effect on. In3, epinephrine with ice water stimulation of the rat model of bloodstasis. Resuts show San Ku dripping pills, high does and middle does group animalblood viscosity and low shear rate of whole blood viscosity reduction（P<0.05）, low dosegroup, low, animal in high shear rate of whole blood viscosity lower than that in the modelgroup（P<0.05.0.01）, the tips of the San Ku dripping pills can reduce blood viscosity, improveblood flow slow state, reduce the extent of myocardial damage.4, onmyocardial ischemia and blood stasis animal effects, San Ku dripping pills may reduceanimal model myocardial CK and LDH contents, serum thromboxane （TXB₂）content and TXB₂/6-k-PGFIalpha ratio, reduce animal serum MDA content,elevated serum SOD content（P<0.05）, the tips of the San Ku dripping pills mayby reducing the levels of thromboxane（TXB₂）, TXB₂/6-k-PGFIalpha ratio, increasethe oxygen free radical scavenging ability, reduce free membrane lipid peroxide（LPO）,level mechanism, improving myocardial local blood circulation, inhibit plateletaggregation, reduce the cells from free radical attack severity of myocardial injury,protective effect.Conclusion: The experimental results show that, San Ku dripping pills may actthrough inhibition of arterial, venous thrombosis, improving blood viscosity,regulating TXB₂/6-k-PGFIalpha ratio, resisting free radical damage, inhibitionof platelet aggregation, myocardial injury on myocardial injury plays certainprotective effect, San Ku dripping pills for clinical treatment of coronary heart disease,angina pectoris and provide the reliable theory basis. Its exact mechanism ofaction remains to be further studied.