The Relations Between Heparanase With Invasion And Metastasis Of Human Beast Tumor And The Effects Of Ulinastatin

Objective:To study the relations between Protein Heparanase(Hpse)、Hyaluronidase-1(HYAL1)and Cellular adhesive molecular CD44v6andinvasion and metastasis of human breast cancer cell MCF-7(the estrinreceptor is positive) and MDA-MB-231(the estrin receptor is negative)andthe effect of ulinastatin.Methods:The breast cancer cell line MCF-7and MDA-MB-231cultured in vitro were treated in randomly divided:1.Control,2.UTI at thelow-dosage group,3.medium-dosage group,4.high-dosage group,5.moderate dosage of UTI with Taxotere(TXT).the proliferation ability ofcells are tested by MTT assay. Western blotting applications are used tomeasure the expression of Hpse、HYAL1and CD44V6;The invasion abilityof the cell lines are tested by Transwell chamber assay with Matrigel;Themigration of the two cell lines are examined by Transwell chamber.Results:The expression of Hpse、 HYAL1and CD44V6wassignificantly decreased compared with the control group, and the effect ofthat received two medicament simultaneously is superior to that UTI alone(P﹤0.05); compared with the control cells,UTI inhibit the invasionand migration of the breast cancer cell MCF-7and MDA-MB-231,moresignificant joint UTI and TXT (P﹤0.05).Conclusions:UTI can inhibit the invasion and metastasis of the breastcancer cell,the mechanism may be associated with UTI reduced theexpression of Hpse、HYAL1and CD44V6.