| Objective:To investigate the effect and the mechanism of Sang-qi Granula in decreasing blood pressure and attenuating myocardial remodeling in Spontaneous Hypertensive Rats(SHR) associated with PPARs (Peroxisome Proliferator-Activated Peceptors) signal pathway. Methods:64male rats with spontaneous hypertension aged4weeks were employed and divided into modeling group (SHR group), Sang-qi Granula+Amlodipine Besylate Tablets group(SHR+LHX+SQ group), Amlodipine Besylate Tablets group (SHR+LHX group), Sang-qi Granula group(SHR+SQ group).16male Wistar-kyoto rats(WKY) rats were employed as control group (WKY group). Rats in each group were sacrificed at two time points (the8th week-treatment and the16th week-treatment). Systolic blood pressure, diastolic blood pressure and mean blood pressure were measured by tail-cuff method. Heart weight index (HWI) was counted. Masson was used to observed cardial fibrosis. Enzyme-linked immunosorbent assay and Western blot were used to determin the expression of ICAM-1ã€TNF-α and PPARs protein. Results:Compared with modeling group, mean blood pressure of groups receiving treatment were all decreased (P<0.05). Effect of16-week C group in decreasing blood pressure was prominent. Compared with control group, HWI of groups receiving treatment were all decreased (P<0.05). Effect of16-week C group and16-week E group in lowering HWI.is outstanding. Compared with model group, ICAM-1and TNF-α expression of groups receiving treatment were decreased (P<0.05). Effect of16-week C group and E group is much bet ter than other groups. Express ion of PPARs increased in groups receiving treatment. Conclusion:Sang-qi Granula plays an important role in decreasing blood pressure, attenuating myocardial fibrosis and protect ing myocardial cell. The longer the treatment cycle is, the better the effect will be. The probable mechanism is that PPARs inhibit excessive expression of cardial inflammtory factors. |
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