| Objectives: this study using the high sugar and high fat feed copy STZdiabetic animal model in rats, and applied to Xiezhuojiedu Huoxuetongluotraditional Chinese medicine with interventional treatment was carried out onthe model, by looking at the biochemical indicators and kidney tissues of ratsthe expression of Hpa, explore the Xiezhuojiedu Huoxuetongluo traditionalChinese medicine with side effect on the treatment of diabetic nephropathyand its possible mechanism, which provide experimental basis for clinicaltreatment of diabetic nephropathy and theoretical basis.Methods: choose85healthy male Sprague wrote-Dauley rats, ordinaryadaptive feed feed a week later, the blood sugar and urine protein was detectedin all negative for used in experiments. Were randomly divided into normalgroup (NG)15, only made70module, normal group gives ordinary feed,building modules to give high sugar, high fat feed. After12weeks, buildmodule fasting rats water, one-time intraperitoneal injection of STZ (35mg/kg). Normal group rats injected with the corresponding volume citratebuffer. Venous blood test back72h random blood glucose, blood sugar orgreater tendency for16.7L model success for our DM. Death in the process ofbuilding two, otherwise a blood sugar not up to standard, all shall be excluded.Will ChengMo rats were randomly divided into model group (MG), Chinesemedicine group (mid-december), western medicine group (er bei sha Tanzania)(XG), the Canadian side group (traditional Chinese medicine combined hesand temple)(JG). Each treatment group given drug lavage, normal group andmodel group suit quite amount of saline solution, each for16weeks.Respectively for4weeks,8weeks,12weeks monitoring body weight, bloodsugar and urine protein. Weekend16,12h after fasting, to return each24-hour urine of rats, urine protein quantitative determination of24hours. Anesthesiaunder the aortaventralis blood, separation of serum, determine the biochemicalindicators and return kidney tissues in light microscope, electron microscope,immunohistochemical, Real-time PCR test.Results:1general situation of the experimental ratsNormal group rats normal diet, urine normal, muscle plump, color luster,responsive. Groups gradually after the injection of STZ rats appeared moredrink, more food, more urinary symptoms, the experiment last week,compared with normal group, making module and the dosage of ratunresponsive, listlessness, small size, the symptoms of model group rats thanthe severe symptoms of each dosage group of rats, the symptoms of traditionalChinese medicine and western medicine group rats severe symptoms thanCanada group. Different stages in the process of experiment, a dead rat. Within72hours after STZ injection, were killed,2rats, blood sugar is not up tostandard1rats reason may be associated with individual differences andoperating of rats, and eliminate. To death four medicine during the modelgroup, Chinese medicine group1only death, death2only western medicinegroup, the cause of death may be due to the sugar metabolic disorders causedby high blood sugar eventually lead to multiple organ failure. TraditionalChinese medicine (TCM) in group2, the western medicine group1, theCanadian side group1only alleviate eliminate blood sugar.2groups of rats body weight, weight, right kidney hypertrophy index(right kidney weight/body weight)Compared with normal group, model group and treatment groupsignificantly reduce weight (P<0.05), model group and treatment group andthe weight has no obvious difference between the treatment group (P>0.05);There was no significant difference between groups in terms of right kidneyweight (P>0.05); Compared with normal group, model group and treatmentgroup hypertrophy index obviously high (P<0.05), model group and eachtreatment group and there was no significant difference between the treatment group (P>0.05).3groups of rats with the comparison of glycosylated hemoglobinCompared with the normal group, model group and treatment groupglycosylated hemoglobin were significantly increased (P<0.05), the Canadianside group and Chinese medicine group is lower than western medicine groupand model group (P<0.05), no obvious difference between Ghana and Chinesemedicine group (P>0.05), no significant differences between model group andwestern medicine group (P>0.05).4groups of rats with24h urine protein quantitativeQuantitative levels in each treatment group24hours urinary protein weredifference compared with normal group and model group (P<0.05), signif-icantly higher than the normal group, significantly lower than that of modelgroup; Comparison between each treatment group, the Canadian side group of24hours urinary protein quantitative lower than that of traditional Chinesemedicine and western medicine group (P<0.05); There was no differencebetween western medicine and Chinese medicine group (P>0.05).5groups of rats serum total protein and albuminEach treatment group serum total protein and albumin were differencecompared with normal group and model group (P<0.05), lower than thenormal group, higher than the model group; The comparison betweentreatment group, the Canadian side group of serum total protein and albuminhigher than that of traditional Chinese medicine and western medicine group(P<0.05); There was no difference between western medicine and Chinesemedicine group (P>0.05).6comparison between groups in the rat kidneyThe normal group, model group and treatment group rats serum creatinineand urea nitrogen levels between no significant difference (P>0.05).7comparison between groups of rats blood lipidCompared with normal group, model group and treatment groupcholesterol and triglyceride levels in rats rats significantly higher than normalgroup (P<0.05), compared with model group, the treatment group of choles- terol and triglyceride levels decreased significantly (P<0.05); Between thetreatment group, compared to the Canadian side group of cholesterol andtriglyceride levels were lower than that of traditional Chinese medicine andwestern medicine group (P<0.05); There was no difference between westernmedicine and Chinese medicine group (P>0.05).8Kidney pathological morphology observation8.1Light microscopyNormal group of normal rat renal tissue structure, structure of glomerularcomplete and clear and no obvious hypertrophy, glomerular capillary basementmembrane is normal without thickening, and mesangial matrix has no obviouschange. Model group rats visible glomerular hypertrophy, glomerular capillarybasement membrane thickening obviously, mesangial cell proliferation andmesangial matrix thickening obviously. Histological comparison between eachtreatment group, the Canadian side group is slightly lighter than the traditionalChinese medicine and western medicine group, no significant differencebetween the traditional Chinese medicine and western medicine group.8.2electron microscopeNormal group organization structure clear, normal rat kidney glomerularcapillary basement membrane evenly, did not see thickening, uniformdistribution of foot processes, no fusion and missing; Visible model group ratssignificantly thickening of glomerular basement membrane, uneven thickness,protruding shows hummocky, foot process fusion, highly vascular endothelialcell proliferation. The treatment group have different degree of pathologicalchanges.8.3Real-Time PCRCompared with normal group, build module Hpa expression in rat kidneyvolume increased significantly (P<0.05); Compared with model group, thetreatment group, the expression of Hpa quantity decreased significantly (P<0.05); Comparison between treatment groups, the Canadian side group, theexpression of Hpa amount less than the traditional Chinese medicine andwestern medicine group (P<0.05), the traditional Chinese medicine and western medicine group had no significant difference (P>0.05).8.4immunohistochemicalNormal group did not see obvious expression of Hpa positive staining, ora small amount of expression; Compared with the normal group, build moduleHpa expression in rat glomerular volume increased significantly (P<0.05);Compared with model group, the treatment group, the expression of Hpaquantity decreased significantly (P<0.05); Comparison between treatmentgroups, the Canadian side group, the expression of Hpa amount less than thetraditional Chinese medicine and western medicine group (P<0.05), thetraditional Chinese medicine and western medicine group had no significantdifference (P>0.05).Conclusion:1Xiezhuojiedu Huoxuetongluo traditional Chinese medicine withChinese traditional medicine can reduce the HbA1c of DN rats, TC, TG,reduce the discharge U Pro, increase the TP and propagated, kidneypathological damage in the rat model of diabetic nephropathy and delay theprogress of DN.2Xiezhuojiedu Huoxuetongluo traditional Chinese medicine withChinese traditional medicine can reduce the expression of Hpa, slow progressin DN, so as to achieve the DN rat kidney protection.3The results showed, traditional Chinese medicine and western medicinegroup similar therapeutic effect, Ghana group than the treatment effect oftraditional Chinese medicine and western medicine alone group, It has aguiding significance for clinical applications. |
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