Expression And Significance Of Sperm Associated Antigen-9in The Tissue Of Cervical Adenocarcinoma

Background Cervical cancer is one of the most common gynecologic malignant tumor, primary cervical carcinoma can be divided into squamous cell carcinoma and adenocarcinoma, majority of the patients are former pathological type. But in recent years, the incidence of cervical cancer increased, and the patients became much younger. Cervical adenocarcinoma and cervical squamous cell carcinoma have significant differences in etiological factors, pathogenesis, clinical manifestations, treatments and prognoses. Cervical adenocarcinoma originate from the cervical canal, in the early stage lesions are barely perceptible, the appearance of the orifice of the cervix can be relativly normal, gynecological examination often can not detect the leisions. Now the main screening method of cervical cancer is TCT (Thinprep cytologic test), however to the cervical adenocarcinoma, the negative result is common thus the disease is easy to be missed. Primary cervical adenocarcinoma is easy to be confused with primary endometrial adenocarcinoma, especially when both the cervix and endometrium have lesions. To the patients of cervical adenocarcinoma, the surgical injury and side effect of the chemo-therapy is great. Besides compared with cervical squamous cell carcinoma, cervical adenocarcinoma has poor prognosis. So early diagnosis, differential diagnosis and prognostic evaluation of cervical adenocarcinoma has important significance. Nowadays there is a lack of an effective molecular marker which can effectively assist the early diagnosis, differential diagnosis, prognosis and targeted therapy of cervical adenocarcinoma. For the incidence rate of cervical adenocarcinoma is low, there’s little research about it, so now exploring a new and effective molecular marker which can assist the early diagnosis, differential diagnosis, prognosis evaluation of cervical adenocarcinoma has become a hot topic.Objective Make an investigation on the expression of sperm associated antigen9(SPAG9) in the cervical adenocarcinoma tissue and the potential clinical application value of SPAG9in the early diagnosis, differential diagnosis, prognosis evaluation of cervical adenocarcinoma, provide scientific basis for targeted therapy of cervical adenocarcinoma.Methods The method of immunohistochemistry staining was applied to determine the expression of SPAG9on the level of protein in the tissue obtained from50patients with cervical adenocarcinoma and50patients without any cervical lesions. The immunohistochemistry H-score method was used to analyze the outcomes of the immunohistochemistry staining.Results1In this study the average first labor age between two groups were similar, the number of patients who had first delivery under21years old in cervical adenocarcinoma group is more than the number in normal cervix group, but the difference is not statistically significant (P>0.05). The number of patients who had conception more than4times in cervical adenocarcinoma group, is greater than that in normal cervix group, but the difference is not statistically significant (P>0.05). In this study the pathogenesis of cervical adenocarcinoma isn’t correlate with the age of first delivery and the times of conception.2Immunohistochemical results show that the rate of expressions of SPAG9in the tissue of cervical adenocarcinoma is100%(50/50), however, in the normal tissue is0(0/18). Results analyses revealed that SPAG9expression was significantly associated with tumor grades.3The expression level of SPAG9is closely related to the prognosis of cervical adenocarcinoma patients.4SPAG9expression levels in different pathological types are different. However for the number of cervical adenocarcinoma cases in this study isn’t enough for a statistical analysis, SPAG9being used as a diagnostic marker of cervical adenocarcinoma in pathological types need to be further investigated.5The SPAG9H-score is higher in the group of patients who take oral conceptive pills than patients who not, but the difference is not statistically significant(P>0.05).Conclusions The extent of the expression of SPAG9in the cervical adenocarcinoma tissue indicates that it may play an significant role on the genesis and progression of cervical adenocarcinoma. SPAG9has the potential of becoming a biological marker of the diagnosis, the prognosis forcast of the cervical adenocarcinoma or becoming a target for molecular target therapy.