Effect Of Compound Danshen Dripping Pills On Intimal Hyperplasia After Angioplasty

ObjectiveIn recent years, vascular intimal hyperplasia and restenosis after angioplasty hasbecome an important problem facing the cardiovascular interventional procedures，Many researchers are trying committed to finding the ways of drugs and methods tosolve this problem。This study uses the abdominal aorta balloon injury in rabbitscombined with fed high-fat diet ways to build a rabbit model of atherosclerosis,thenwe use the vascular dilatation balloon again established animal model of restenosisafter angioplasty。 Then model rabbit were given compound danshen drippingpills(CDDP)after surgery intervention,Finally we observed the effect of CDDP forrestenosis after angioplasty and to explore its possible mechanisms.Methods38male New Zealand white rabbits（weight2.0-2.5kg) of clean grade were selected,divided into sham operation group (3rats) and atherosclerosis group (35rats), thesham operation group received normal diet, model group were fed with high fat diet(150mg/d), one weeks later, The sham operation group underwent sham operationabdominal aortic,The model group underwent balloon injury of the abdominalaorta,then the sham group to normal diet, model group to high fat diet;After fourweeks, the model group were randomly selected3comparison with the sham group todetermine atherosclerosis model was established; then the model group underwentballoon angioplasty, restenosis after angioplasty established model. Restenosis modelgroup were randomly divided into a control group (A)(2ml/kg/d saline gastric lavage+normal diet), CSDP low dose group (group B)(CSDP300mg/d lavage+normal diet), CSDP high dose group (group C)(600mg/d lavage+normal diet), amlodipinebesylate group (group D)(5mg/kg·d lavage+normal diet).After6weeks, the animalswere sacrificed and the abdominal aorta sections stained with HE and bFGF, PCNA,TGF-β1enzyme immunohistochemical staining and evaluated for expression levels.Respectively, in the experimental animal model of restenosis success and each end ofthe experiment time detection of blood total cholesterol (TC), triglyceride (TG), lowdensity lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C)levels.Results1.Lipid parameters: Modeling success, A group (control group), group B (lowdose CSDP), group C (high dose CSDP), D group (amlodipine besylate group) serumTC, TG, LDL-C levels were significantly elevated, HDL-C decreased, there were nosignificant differences between the groups(P>0.05).End of the experiment, nosignificant differencein group A TC, TG, LDL-C, HDL-C levels compared withmodeling (P>0.05), while three drug groups TC, TG, LDL-C compared with group Adecreased significantly, HDL-C rise (P <0.05), TG between the three groups treatedgroup, LDL-C, HDL-C was no significant difference (P>0.05); high dose CDDPgroup (group C) TC compared with amlodipine besylate group (group D) decreasedsignificantly (P <0.05).2.The vascular morphology:The control group (group A) vascular visible stenosis,endometrial thickening, collagen fiber hyperplasia and deposition.Three treatmentgroups (including group B, C, D group) compared with group A,Lumen area (LA)increased significantly, the intima area (IA), medial area (MA), the stenosis rate wassignificantly reduced (P<0.05).The lumen area (LA) in Group D was increased moresignificantly than group B (P <0.05), but comparing with B、C groups，the intimal area(IA)、medial area (MA) and stenosis were not statistically significant (P>0.05).3.bFGF, PCNA, TGF-β1immunohistochemistry images:Expression of bFGF andPCNA-positive cells in the control group (A) focused on the luminal side of theintima close to significantly reduce low CDDP group (group B), high CDDP group(group C) compared with group A, positive cells distributed significantly decreased (P<0.05), B group compared with C group, no significant difference (P>0.05). Controlgroup TGF-β1expression in endometrium, group B and group C, respectively,compared with group A, the expression of positive cells was significantly increased (P<0.05), group B compared with group C, no significant difference (P>0.05).Compared with CDDP, amlodipine besylate in reducing postoperative cell bFGF, theexpression of PCNA better (p <0.05), but the increase in the level of TGF-β1effect isquite (p>0.05).Conclusions1.CDDP can significantly reduce the serum TC, TG, LDL-C levels, increased HDL-Clevels, thus delaying vascular development of atherosclerosis, high doses of CDDPreduced the effect of TC compared with amlodipine may be more pronounced.2.CDDP can reduce vascular endothelial cells after balloon injury bFGF, PCNAexpression, inhibition of neointimal proliferation after postoperative.3.CDDP after balloon injury can upregulate the expression of TGF-β1in vascularendothelial cells, inhibition of vascular smooth muscle cells transformed phenotype,thereby inhibiting smooth muscle cell migration and proliferation.