Correlation On MRNA Expressions Of Transcription Factors RORγt And Foxp3in Peripheral Blood Mononuclear Cells Of Patients With Psoriasis Vulgaris

Objective:To explore the peripheral blood of patients with psoriasis vulgaris Th17cells,Treg cells specific transcription factor RORyt, Foxp3mRNA expression analysis of its correlation with the incidence and severity of the disease.Method:Seventy-eight cases of patients with psoriasis were selected as a research object, gave the study stage and the Psoriasis Area and Severity Index(PASI) score,60cases in the control group were healthy. Peripheral blood mononuclear cells (PBMCs) in RORyt,Foxp3mRNA expression levels were detected by Real-time fluorescent quantitative reverse transcription-polymerase chain reaction(QRT-PCR).Results:RORyt, Foxp3mRNA in peripheral blood of psoriasis vulgaris group relative expression ratio of the amount of volume and RORγt/Foxp3expression was7.67±0.72,1.63±0.34,4.68±0.61,according to the normal control group, respectively,the difference was statistically significant (all P<0.01). Results indicate that the amount of RORyt mRNA expression in peripheral blood of the sick group was significantly higher and that of Foxp3mRNA expression was significantly lower than the control group,RORyt/Foxp3the expression of the ratio was also increased in the sick group.Also,the peripheral blood of RORyt mRNA expression in conduct period was obviously higher than the static period and Foxp3mRNA expression was significantly lower than the quiescent.RORyt/Foxp3expression of the ratio during the period was significantly higher than resting.The RORyt mRNA relative expression amount and RORyt/Foxp3expression of the ratio of positive correlation had a positive correlation with the PASI score of conduct period (respectively, r=0.672,0.743, all P <0.01). However, Foxp3mRNA relative expression level was negatively correlated with the conduct period PASI Score (r=-0.795, P<0.01). The quiescent PASI score and RORyt mRNA relative expression of the volume and RORγt/Foxp3expression of the ratio was positively correlated (r=0.771,0.816, all P<0.01), relative expression levels of Foxp3mRNA was negatively correlated (r=-0.863, P<0.01).Conclusion:The pathogenesis and severity of psoriasis vulgaris has a relationship with mRNA expression of RORyt increasing and Foxp3descending in peripheral blood.The imbalance of the two gene expression may be an important reason causing Th17/Treg imbalance in peripheral blood of psoriasis vulgaris.