Honey Polygala Quality Standard And Comparative Study With Polygala

Objective:To study the establishment of honey Polygala quality control standards; and to explore the differences that the honey Polygala and raw Polygala may exist on the main ingredient.Methods:Orthogonal test was employed for the honey preferred process by the indexes of the total onjisaponins,alcohol-soluble extractive and appearance composite score.four factors of affecting the honey process,namely the added amount of honey,braised embellish temperature time,making time,making investigation.The L9(34) orthogonal table was used to arrange experiment.Using the thin-layer chromatography(TLC) to identify the tenuifolin and Polygala xanthone Ⅲ.Using the high performance liquid chromatography(HPLC) to determine the tenuifolin,Polygala xanthones Ⅲand 3,6’-two mustard acyl sucrose content. And compares honey Polygala with raw Polygala on these three different components.Using the thin layer chromatography(TLC) to compare honey polygala and raw polygala on the area of petroleum ether, ethyl ether and n-butyl alcohol.Results:Polygala honey roast optimal processing technology as follows: 20 percent plus refining honey, stew Run 6h,frying time of 12 min,frying temperature is 60±5℃.Validation test results showed that The optimization honey processing technology of Polygala is reasonable and reproducible,and can provide the concrete optimized parameters,and the technology basis for honey processing technology of Polygala.The relevant spots of tenuifolin and Polygala mouth xanthone Ⅲ were determined,and plates were clear,and behaviors were better.and can used as a qualitative identification method.tenuifolin and Polygala xanthone Ⅲand 3,6’-acyl sucrose content was determined by HPLC,which was simple,accurate,reliable and reproducible,can be used to control the quality of honey Polygala tenuifolia. Among them,tenuifolin content was determined by the "Chinese Pharmacopoeia" 2010 version of "raw Polygala" methodologies;Polygala mouth xanthone Ⅲand 3,6’-acyl sucrose content determinationmet method is improved:HPLC analysis was performed on Column elite ODS-BP(250mm×4.6mm,5μm), acetonitrile-0.05% phosphate gradient system was 0~30min(A is 15%),30~50min(A is 15%~30%),50~52min(A is 30%~100%),52~60min(A is 100%), 60~62min(A is 100%~15%),62~75min(A is 15%),Flow rate:1.0m L/min;detection wavelength: 320 nm.The results can be seen from the TLC at 365 nm and sunlight, the chemical composition of parts of n-butanol between honey Polygala and raw Polygala has no significant difference. But in parts of petroleum ether and ether, the difference is large, some spots strengthened,somewhat weakened, or even disappeared.Conclusion:the honey processing technology of Polygala was Specificated.Process parameters were optimizated.To ensure uniform quality and stability of the honey Polygala. And provided experimental evidence of science for clinical use and production. A reasonable and feasible quality standards of the honey Polygala wasestablished.Among them,TLC plates were clear, and behavior better,the method o f three ingredients conteng determination is simple and fast,and developed a reasonable limit. Building a Polygala xanthone Ⅲand 3,6’-two mustard acyl sucrose content determination meth-od is accurate good reproducibility, and durability.After Polygala honey-roasted, the main component content are slightly lower, but not significantly different, but compare different polarity by TLC, the differences still exist in weakly polar parts, the cause of difference component formed and the affect of its efficacy remain to be further studied.