Studies On The Chemical Constituents And Anti-H5N1 Activity Of The Stems And Leaves Of Buddleja Lindleyana Fort

The avian influenza A(H5N1) virus subtype, a highly pathogenic AI(avian influenza) virus, first infected humans in 1997 during a poultry outbreak in Hong Kong SAR, China. Since its widespread re-emergence in 2003 and 2004, this avian virus has spread from Asia to Europe and Africa and has become entrenched in poultry in some countries, resulting in millions of poultry infections, 846 human cases, and 449 human deaths(data source: WHO/GIP, 20 January 2016). Outbreaks in poultry have seriously impacted livelihoods, the economy and international trade in affected countries. China, the world’s most populous country, has maintained keen vigilance at its epidemic situation and variation.According to previous research, seven species of Chinese herbs were got to show cytoactive screened from infected MDCK cell lines, judging by the cytopathic effect(CPE) method. I chose the Chinese Traditional Medicine- the stems and leaves of Buddleja lindleyana Fort. to study after research and repeated comparisons.Distributed widely in South China, Buddleja lindleyana Fort. is one kind of deciduous shrub affiliated to Buddleja in Buddlejacceae. Its stems and leaves were used as Traditional Chinese Medicine in treating numps, scrofula, dorsal furuncle, ancylostomiasis, fishbone sticking and wind-cold toothache, clinical treatment in bronchial asthma as reported. Also, its flowers and roots can be used as drugs in eliminating phlegm, detoxicating, activating blood and dispersing stagnation too. So far, few papers were found to report its chemical constituents, neither were its anti-H5N1bioactivity. Consequently, this work performed systematic analysis on its chemical components, combined with cytoactive detection to screen activity ones.Twenty-three compounds were isolated and purified via bioactivity-guided fraction-ation, utilizing chromatography such as AB-8 macropore resin, silica gel, Sephad-ex LH-20, ODS C18, preparative thin layer chromatography(PTLC), high performance liquid chromatography(HPLC), preparative HPLC, and recrystallization. St-ructures were identified according to the physicochemical property and spectra d-ata, shown as follows: linarin(1), apigenin-7-O-α-L-rhamnopyranosyl-(1â†'2)-β-Dglucopyranoside(2), rutin(3), hesperidin(4), apigenin-7-O-β-D-glucopyranoside(5), hyperoside(6), apigenin(7), luteolin(8), hesperetin(9), quercetin(10), daidz-ein(11), salicylic acid(12), salidroside(13), syringate(14), benzoic acid(15),(Z)-6-docosenamide(16), oleanolic acid(17), β-sitosterol(18), daucosterol(19). C-ompounds 4-6 and 12-16 were isolated from this genus for the first time. Comp-ounds 2-3, 7-10 and 17 were obtained from this plant for the first time.Compounds were tested for cytotoxicity against the MCF-7, Hela and HT-29 cell lines in vitro using the MTT assay method. Compound 10 exhibited potent toxi-city effects against the Hela cell lines, with IC50 values of 28.6425 μg/ml.