From The Gut MUC-2, AQP3 Through The Expression Of Nitric Turnip Square Knot Modified Treatment Of Functional Constipation And Mechanism Of Action

Objective: To investigate the effect of nitric turnip through catharsis square knot different prescriptions and intestinal MUC-2 expression levels and the impact AQP3 of functional constipation in rats through analysis of nitrate turnip square knot Modified treatment of functional constipation and mechanism of action, In order to optimize the composition of prescriptions, improved medicines form the foundation.Methods:During the collection of adult SD rats were fed a solution of compound diphenoxylate way to create functional constipation model, modeling fecal output, fecal calculated moisture content, the recording time and observe the changes: methods. After the modeling, giving nitro turnip pass junction Modified Decoction gavage. After treatment, he collected colon samples, HE staining pathological changes of the colon morphology, immunohistochemistry to detect MUC-2, AQP3 factor protein expression, Q-PCR method to detect MUC-2m RNA, AQP3 m RNA expression.Results:(1) General behavior: the rats have appeared during the modeling, eyes dull, lazy move, get together, such as slow excitability decreased mental performance, brown hair, hair removal, most dry stool like sheep feces, such as strings beads, fewer bowel movements, stool black, full, induration, yellowish urine, weight loss, slow growth rate, muscle thin, soft skin and muscle. After modeling the end of the week, the symptoms improved. After treatment, the symptoms returned to normal.(2) Weight change: Compared with model group, nitro turnip knot growth through value addition and subtraction party body weight of rats was significantly higher(P <0.05), plus or minus square knot nitrate turnip through group compared with the control group, the difference is small, no significant difference(P> 0.05).(3) The amount of change in rat feces: Compared with model group, nitro group turnip through knot plus or minus square stool volume increased(P <0.05), plus or minus square knot nitrate turnip through group and the control group, the difference is small, no statistically significance(P> 0.05).(4) Rat feces moisture content: Compared with model group, nitro group turnip pass stool square knot subtraction amount higher water content(P <0.05), plus or minus square knot nitrate turnip through group compared with the control group, the difference is small, no significant difference(P> 0.05).(5) Histopathological changes: Compared with model group, nitro group turnip through square knot subtraction Histopathologic big difference, plus or minus square knot Nitrate turnip through group compared with the control group, no significant difference.(6) Colon tissue MUC-2, cytokine expression AQP3 protein: Compared with model group,nitro group turnip through square knot subtraction MUC-2 expression was significantly increased, AQP3 expression was significantly increased, nitrate turnip through subtraction square knot group and the control group comparison, no significant difference.(7) Colon tissue MUC-2m RNA, AQP3 m RNA expression: Compared with model group, nitro group turnip through square knot subtraction MUC-2m RNA relative expression were significantly increased, AQP3 m RNA relative expression was significantly increased, nitrate turnip through subtraction square knot group compared with the control group, the difference was small(P> 0.05).Conclusion: Diphenoxylate enteric-coated tablets successful replication functional constipation model rats. Glass turnip through subtraction junction intragastric treatment of constipation model rats significantly increased body weight, increased fecal excretion, improve its moisture content, but with time, the effect gradually obvious, and stool texture becomes normal colon tissue morphology returned to normal, improved MUC-2, AQP3 protein and m RNA expression levels, improve water metabolism disorders. The experiment proved that nitric turnip through subtraction square knot groups, namely the original party plus Atractylodes, subtract 1/3 Glauber’s salt, 2 times peach, 2x betel nut having a good cathartic effect, and because Atractylodes, betel nut and peach are foodborne Drug and non-mineral drugs, it can be used as nitrate turnip square knot through good alternatives to address nitrate turnip through knot pill production stagnation problems.